FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_F04_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_F04_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178883
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTATTAGTAGTAACCGGGTTATTAGTAGAAACACCGGGTTATTAGTAG15130.8458042407607207No Hit
GTAGTAACACCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGT14820.8284744777312545No Hit
GTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTATTAGT11590.6479095274564939No Hit
CAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTATTAGTAGAAACA8250.4611953064293421No Hit
TAGTAACACCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGTA7950.44442456801372965No Hit
GTAGTAACCGGGTTATTAGTAGAAACACCGGGTTATTAGTAGAAACAACG7920.4427474941721684No Hit
TATTAGTAGTAACCGGGTTATTAGTAGAAACACCGGGTTATTAGTAGAAA7750.4332440757366547No Hit
GTAACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTATTAGTAGA6680.37342844205430364No Hit
GTAGTAACAAGAGGATTCCGGGTTATTAGTAGAAACACGTGGGTTATTAG6230.34827233443088496No Hit
TAACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTATTAGTAGAA6060.3387689159953713No Hit
CCCGGAATCCTCTTGTTACTACTAATAACCCGGAATCCTCTTGTTACTAC5670.31696695605507513No Hit
AACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTATTAGTAGAAA5570.31137670991653765No Hit
ATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTAT4830.2700088884913603No Hit
GGTTATTAGTAGTAACACCGGGTTATTAGTAGAAACAACAGCCCGGGTTA4570.2554742485311628No Hit
GTAGAAACACCGGGTTATTAGTAGAAACAACGCACCCGGGTTATTAGTAG4570.2554742485311628No Hit
CAAGAGGATTCCGGGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAG4400.24597083009564913No Hit
GGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACAAGAGG4360.24373473164023413No Hit
ATTAGTAGTAACACCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAG4270.23870351011555038No Hit
CAAGAGGATTCCGGGTTATTAGTAGAAACACGTGGGTTATTAGTAGAAAC4240.23702643627398914No Hit
GGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGTAACCGGGTTATT4150.2319952147493054No Hit
GGCTCTTGTTACTACTAATAACCCGGGCTCGTGTTTCTACTAATAACCCG4130.2308771655215979No Hit
GTAGAAACACGTGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAG4090.2286410670661829No Hit
CAACAGCCCGGGTTATTAGTAGTAACCGGGTTATTAGTAGAAACACCGGG4040.22584594399691418No Hit
CACGTGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACACG3960.2213737470860842No Hit
GGTTATTAGTAGAAACACCGGGTTATTAGTAGAAACAACGCACCCGGGTT3930.21969667324452297No Hit
AGAAACAACAGCCCGGGTTATTAGTAGTAACCGGGTTATTAGTAGAAACA3600.20124886098734926No Hit
GTAGAAACAACAGCCCGGGTTATTAGTAGTAACCGGGTTATTAGTAGAAA3510.19621763946266554No Hit
AGTAGAAACAACAGCCCGGGTTATTAGTAGTAACCGGGTTATTAGTAGAA3460.1934225163933968No Hit
GTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACAAGAGGATTCCGGG3440.1923044671656893No Hit
CCCACGTGTTTCTACTAATAACCCGGAATCCTCTTGTTACTACTAATAAC3430.19174544255183557No Hit
AGTAGAAACAACGCACCCGGGTTATTAGTAGTAACAAGAGGATTCCGGGT3390.18950934409642056No Hit
GGGCTCGTGTTTCTACTAATAACCCGGGCTCGTGTTTCTACTAATAACCC3370.1883912948687131No Hit
TATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGGTTA3270.1828010487301756No Hit
GTAACAAGAGGATTCCGGGTTATTAGTAGAAACACGTGGGTTATTAGTAG3090.17273860568080812No Hit
CCCGGAATCCTCTTGTTACTACTAATAACCCGGGTGCGTTGTTTCTACTA3010.16826640876997814No Hit
GTAGAAACACGAGCCCGGGTTATTAGTAGAAACACGTCCGGGTTATTAGT3000.1677073841561244No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTA2960.1654712857007094No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGTCCGGGTTATTAGT2910.16267616263144066No Hit
GGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACACGAGCCCG2890.16155811340373316No Hit
TAACAAGAGGATTCCGGGTTATTAGTAGAAACACGTGGGTTATTAGTAGA2850.15932201494831816No Hit
ACAACAGCCCGGGTTATTAGTAGTAACCGGGTTATTAGTAGAAACACCGG2840.15876299033446442No Hit
GGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACACCGGG2780.15540884265134194No Hit
TATTAGTAGTAACACCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTA2710.1514956703543657No Hit
TGTTTCTACTAATAACCCGGAATCCTCTTGTTACTACTAATAACCCGGAA2610.14590542421582822No Hit
GAATCCTCTTGTTACTACTAATAACCCGGAATCCTCTTGTTACTACTAAT2590.14478737498812072No Hit
GGTTATTAGTAGAAACAACGCACCCGGGTTATTAGTAGTAACAAGAGGAT2570.14366932576041322No Hit
AACAAGAGGATTCCGGGTTATTAGTAGAAACACGTGGGTTATTAGTAGAA2560.14311030114655948No Hit
GGAATCCTCTTGTTACTACTAATAACCCGGAATCCTCTTGTTACTACTAA2550.14255127653270572No Hit
GGGCTCGTGTTTCTACTAATAACCCACGTGTTTCTACTAATAACCCGGAA2460.137520055008022No Hit
GGCTCGTGTTTCTACTAATAACCCACGTGTTTCTACTAATAACCCGGAAT2440.13640200578031453No Hit
GGGCTGTTGTTTCTACTAATAACCCGGGTGCGTTGTTTCTACTAATAACC2430.13584298116646076No Hit
AAACAACAGCCCGGGTTATTAGTAGTAACCGGGTTATTAGTAGAAACACC2350.13137078425563078No Hit
ATTAGTAGTAACCGGGTTATTAGTAGAAACACCGGGTTATTAGTAGAAAC2300.12857566118636204No Hit
GTAGAAACACGAGCCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTA2290.12801663657250828No Hit
GTTATTAGTAGTAACACCGGGTTATTAGTAGAAACAACAGCCCGGGTTAT2270.1268985873448008No Hit
GGGTTATTAGTAGTAACCGGGTTATTAGTAGAAACACCGGGTTATTAGTA2210.1235444396616783No Hit
CTAATAACCCGGGTGCGTTGTTTCTACTAATAACCCGGTGTTTCTACTAA2190.12242639043397081No Hit
GTGTTTCTACTAATAACCCGGTTACTACTAATAACCCGGGCTGTTGTTTC2180.12186736582011706No Hit
GATTCCGGGTTATTAGTAGTAACACCGGGTTATTAGTAGAAACAACAGCC2160.12074931659240956No Hit
GGGTGCGTTGTTTCTACTAATAACCCGGTGTTTCTACTAATAACCCGGTT2120.11851321813699457No Hit
GGGTTATTAGTAGTAACACCGGGTTATTAGTAGAAACAACAGCCCGGGTT2070.11571809506772583No Hit
ATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGAAACACGTGGGTTA2070.11571809506772583No Hit
CACCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGTAACCGGG2040.1140410212261646No Hit
GGGCTCTTGTTACTACTAATAACCCGGGCTCTTGTTACTACTAATAACCC2040.1140410212261646No Hit
GGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACACGTCCGGG2030.11348199661231084No Hit
GGAATCCTCTTGTTACTACTAATAACCCGGGTGCGTTGTTTCTACTAATA2010.11236394738460334No Hit
ACCCACGTGTTTCTACTAATAACCCGGAATCCTCTTGTTACTACTAATAA2000.11180492277074959No Hit
TATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGAAACACGTGGGTT1990.11124589815689585No Hit
ACACGTGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACAC1980.1106868735430421No Hit
GGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGAAACACGTGG1970.11012784892918835No Hit
GGCTCTTGTTACTACTAATAACCCGGGCTGTTGTTTCTACTAATAACCCG1850.10341955356294337No Hit
AGTAGTAACACCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAG1830.10230150433523588No Hit
GGGCTCTTGTTACTACTAATAACCCGGGCTGTTGTTTCTACTAATAACCC1800.10062443049367463No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)