FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_F02_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_F02_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996016
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT71080.7136431543268381No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA33010.33142037878909575No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT27470.27579878234887795No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT25980.2608391833062923No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT24220.24316878443719778No Hit
CCAATAGGGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT22170.2225867857544457No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC18120.18192478835681355No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17300.1736919888837127No Hit
GTATGGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG16770.16837078922426948No Hit
GAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCGTTT16110.16174438964835905No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATACAGTAACGC15230.15290919021381183No Hit
ATACAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT15030.1509011903423238No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA14540.1459815906571782No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG14020.1407607909913094No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA13890.13945559107484218No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG13450.13503799135756855No Hit
TCCTTGTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTG13300.13353199145395256No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG13170.13222679153748534No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAT13050.13102199161459255No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA12980.13031919165957173No Hit
ATATGGAACTTCTGGTAGTTCATGATTGTAAATGTGAGGCCCTATTGGCC12890.12941559171740213No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA12550.12600199193587253No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG12370.1241947920515333No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGA11860.11907439237923889No Hit
TCTCAGAGCTGACACTATAAGTTCACAAATAGAACTTGCAGTCTTGCTTT11840.1188735923920901No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG11810.11857239241136688No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTCTTCCACTCTGG11750.11796999244992049No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC11650.11696599251417648No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG11620.11666479253345327No Hit
CCTATTTATGAATCCGTCTGCTGGAATTGAAGGATTTGAGCCATACTGTA11560.11606239257200687No Hit
GCTTTATGGTCTGGCGGTTAAAGGACAATCTCATCTGAGAGGAGATACTG11370.11415479269409327No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC11310.11355239273264688No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC11200.11244799280332846No Hit
ATTTACTCTGTCTCCCTTGAAACAAGCTTTGGTCATGTCATATCCTTGTA11190.11234759280975405No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA10880.10923519300894764No Hit
TTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACATGACC10750.10792999309248044No Hit
GTACATGACCATGAGACAATACAGTAACGCTGAGCTTTCATGGCCTTCTG10470.10511879327239723No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG10420.10461679330452524No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG10360.10401439334307883No Hit
AGACAATACAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGC10270.10311079340090921No Hit
CCATGAGACAATACAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCA10150.10190599347801642No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)