FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_E11_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_E11_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623019
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC28030.1727028457461065No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA24390.1502755050926699No Hit
ACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAGTCATGTTAAG22000.13554986109219916No Hit
CTAAAGAAGAGCCCTTGTCAAGGTTACCAGCAACCTCCACATTGGCAAAC21740.13394790818838226No Hit
GTTGTAAGGCTTGCATAAATGTTATCTGTTCGAAACTATTCTCTGTCGCT21250.13092884310041966No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG20730.12772493729278586No Hit
TCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGAGG17940.11053475036336605No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA17290.10652986810382381No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA17250.10628341381092889No Hit
CTTCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGA16790.10344918944263745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)