FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_E08_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_E08_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313315
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC106540.4605512003337202No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA71870.3106796955883656No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG63900.2762269729803334No Hit
AGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTCT52270.22595279933774692No Hit
GAAGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATT49900.21570776137274864No Hit
AAGCAGAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC46780.20222062278591546No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA44670.1930995130364866No Hit
AAGCAGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGC41860.1809524427066785No Hit
GCTCAGAGGTGGGCGTGGAGATGGCGTAGCCATTGTTCCGGCAGAAGAAG39110.1690647404266172No Hit
AAGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC38350.1657794117964912No Hit
GCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTCTG38080.16461225557263062No Hit
CTCTTGGACAGGGGAATCAAAAGCTGCTTAGAGTGGTCAGGAGACGTGCC37360.1614998389756691No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT37240.16098110287617554No Hit
GCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCGTGGAGA37230.1609378748678844No Hit
GTGTAGGGACGTGCATGACCTCTGAGCAAGGCAGAGGGTATTGAAGAAAG35500.15345942943351856No Hit
CCTATGTGCTAGGCACACTTTCTAGGTGCTGAGTGGCCTTGGGCAGGTCC35140.15190322113503782No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG34700.150001188770228No Hit
GTTATAAATCTGTGAGTGATTTCATAACCAAACCTCGTGCCTTGTAACTG34280.14818561242200046No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT31720.13711924229947067No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG30810.13318549354497766No Hit
ATACTGGACTCTTCAGCCTGAGCAGTTGGAAGGGTGGAGTTGCTTTCAGC30650.13249384541231957No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT29190.1261825562018143No Hit
CCCTTATAGCAACATGAGTTTAGAGCAAGAATCACACCCCGACTTTACAG28870.12479925993649804No Hit
AGCAGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCG28720.12415083981213107No Hit
AGCACGCACTTGGGCACAACACAGACAAAGGTGGGGGGTCAGTGCCTGGG28100.12147070329808088No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA27720.11982803898301787No Hit
GCTATAAGGGTTACGAGAGCTCATGCCAGGGAAGCACCTGGTACACAGTA27000.11671562238605637No Hit
CTTTCTAGGTGCTGAGTGGCCTTGGGCAGGTCCCTTACTTCTCTGAGCCT26410.11416516989687958No Hit
GTGTAGTGCAGTGTCAGTAGTGTGGGCTTGAGGCCTGGCTTTTCACCCGT26380.1140354858720062No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC26330.11381934583055053No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT25580.11057724520871562No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC25250.10915072093510828No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT24900.10763774064491864No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC24170.10448209603966602No Hit
GTCCAAGAGGGTAGGCCCCCTGTTCCTATTTACAGGATCCATAAAGAGAC23870.10318525579093205No Hit
TCCTTCGACTGTCCTTGCAGCTGGGATAAGAGTCCCTGACTCTGGTTATG23530.10171550350903356No Hit
GTATTGAAGAAAGCATTGGCCTCTCACTCCCTCATGGGTTCTGCAGGATG23520.10167227550074245No Hit
CCATTACAGTCCCTCAAGATCCATCCCTCCCTCCATGATGCCTGGCTGCA23370.10102385537637545No Hit
GCACTACACCACCTCTCAGGAGAGAAGGCAAATATTTCCTTGACTCAGCC23310.10076448732662868No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT23230.1004186632602996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)