FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_E08_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_E08_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3744695
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA130390.3481992525425969No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT101720.27163761000562126No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG94920.2534785877087453No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA73300.1957435785825014No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA68410.18268510519548323No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG68260.18228453852716975No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG65800.17571524516682935No Hit
GTATAGGAAACTTAAGAGAGAGATAACTTTCCATGGGGCCAAAGAAATAG63550.16970674514212775No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT59490.1588647406531106No Hit
TTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAGAAAAGACGA59170.15801019842737526No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA57230.15282953618385475No Hit
CTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA54480.1454858139314417No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT52680.1406790139116804No Hit
GGTATGTGCAACATGTGAGCAAATTGCTGACTCCCAGCACAGGTCTCATA50970.13611255389290716No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC49950.13338870054837576No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA48430.1293296249761329No Hit
CCTCTTTCTTAATCCTTCCAGACTCGAAATCAATTCTGGCATCAATCCGG48270.12890235386326523No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT48140.12855519608406024No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA47120.12583134273952884No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG47040.12561770718309503No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT46730.12478986940191392No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG46510.12420237162172086No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA46460.12406884939894972No Hit
GTATTTCTCCCATTTATGTGGCTCAAGTCTCGGGTCAGTGAGAGAAAACT45710.1220660160573825No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCCCTTTTAAGGCCGTG45510.12153192716629793No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA45480.12145181383263523No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAGAAAAGACG45300.1209711338306591No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT44830.11971602493661034No Hit
GTACGTGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT43410.11592399380990975No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC42070.11234559823964302No Hit
GTGCTGGGAGTCAGCAATTTGCTCACATGTTGCACATACCAGGCCAAATG41710.11138423823569076No Hit
ATCTACAAGTCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT41140.10986208489609968No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC40600.10842004489017129No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT39990.10679107377236331No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC39070.10433426487337419No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT38990.10412062931694037No Hit
GTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTC38630.10315926931298809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)