FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_E05_FluA_Rep1_Plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_E05_FluA_Rep1_Plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCATAAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA11.8518518518518516No Hit
GGTAAAGATTTATGCTGTACCATGATTTTAGTCATATGGGATAATCAACT11.8518518518518516No Hit
AGCTACAATTGGACTGGAGTCACTCAAAACGGAACAAGCTCTGCTTGCAT11.8518518518518516No Hit
CTTATGATCTTATGTCTAAAAAATCAAAACAACATTATCAATTGATTTCA11.8518518518518516No Hit
GTTCAGGTTCAGGTCCAGATGAAGCAAGGGAAGTCTGGATACACCCCCCA11.8518518518518516No Hit
CTTGATCCATCATTGCCCTCAGGGCAGCTGTTTGAAATTTTCCTTTGAGG11.8518518518518516No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT11.8518518518518516No Hit
ATAGTGAAAACAATCACGAATGACCGAATTGAAGTTACTAATGCTACTGA11.8518518518518516No Hit
ACACTAAACGTGACGTCTCGTTGATCGTTCTTCCCATCCACACGTCATTT11.8518518518518516No Hit
GTCTAAGGTCCAAGCATTGATATGTATGAAAAATTCCCAAGGTGATTCTA11.8518518518518516No Hit
ATATTAAGCTACAATCAAGACTACTCGTTAAGTAATGAATACTCATTGCA11.8518518518518516No Hit
CGACATTGTCAAACACTAAAGCCGCAACCAAGACCACACCTACCATCACC11.8518518518518516No Hit
CCAATAACCGCATAAACATCTTCGAAGCTTATAAGATCCGAATCTCACCA11.8518518518518516No Hit
GCACAAAACAGGCCAAGTGACACTTCTGTGGTAACAGCCCCCATCCTATT11.8518518518518516No Hit
GTAAAAAACTACGAACGTCAGATTGAATCATTTACATATCAATCTTCACA11.8518518518518516No Hit
AAAAAGGAATGGCCCCTTAAGAATTTTAGCCAAACAATTAAGTCAATTAT11.8518518518518516No Hit
GCATGGCATCCACCACTGACTCCATCAGCCTCTCTCAGGGCACCATCGTC11.8518518518518516No Hit
AATAAAAAAACTACAACTCTTCACCCATCAAACGACAATAAATAAAGGAT11.8518518518518516No Hit
GTATAAGTTATCTAGTTATCCCAGAGAAAACTACCATAAACTCTGTGGTA11.8518518518518516No Hit
GACAATAACTCCAAAGAGAGAGCTATACCGAGAAAGATCCGGCACGGCAC11.8518518518518516No Hit
CCAACTCTCCTCCTTATTTTAACTTGATTTCTGAAGTGGACAGGGCCGAA11.8518518518518516No Hit
CCACCTAGCCAAACCCTAAGGCCTCTTGGATGTGCTCACGATCCCTGCCC11.8518518518518516No Hit
TGCCTGGCCTGACTAGCAATCTCCATGGCCTCCGCTGCCTGCTCACTTGA11.8518518518518516No Hit
GTCCATTCCTTACCATCATGGCAAGCAGACCCGCTCCATGCTGACATGTG11.8518518518518516No Hit
CATTAGTACAGAAGGAGAAGACCAAATTACCCATTGGCGTTTCCTCACTC11.8518518518518516No Hit
AACAACTAATGTGCACCAATGTTCCAATGTATCCACATGCTCCTATCTGA11.8518518518518516No Hit
CAATGGGACAAATCTGCTGTTAAATAATTACTTCTCATCCTTGCAATGTT11.8518518518518516No Hit
GACCCCTGGGTCCAGAGCAATTATTTGGACCATGAAACAACTCAAACTAC11.8518518518518516No Hit
CCATGAGTCCACTTTCTCAAATTCCGTTCACCCAAATCAAGAGTAATTCT11.8518518518518516No Hit
GGTAAGAGCAAATCAGAATCGAGCCCAAAATAGCATCAGATATAAAAATA11.8518518518518516No Hit
GTTTGTCATTTAGCAAGGCCCCTGGAGTCAAGAAGAAGGTTCTGCATTCC11.8518518518518516No Hit
GACTTCTATGGTGTTGGCTAATGCAGTTGCTGCCGGTTGATTTCTGTTTA11.8518518518518516No Hit
CAATAAAGAAGTATATTTCATTAAAAAAGTATTTACTTCTATCACTCCCT11.8518518518518516No Hit
TGGAAAGGGCATGAGCTAGACAAATGGCAATAATGAGGAAAAGAGGAGGC11.8518518518518516No Hit
AACCACGCAAACGAGCCTGACAAGGAATGGAAAGAGATCAGCCAGGAGAA11.8518518518518516No Hit
CGTATGTGCAACAAGTGAGCAGATTGCAGATTCCCACCACAGGTCTCATA11.8518518518518516No Hit
GGTCACTCAGTGGCAGGGTCAGGCTAAGAAACCTGGTGTTCCAATTTCCT11.8518518518518516No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC11.8518518518518516No Hit
AGCAAAAGCAGGGCAGCACGTGCTAGAGCCTGGCATATAAGATGCTCTCC11.8518518518518516No Hit
GTGTTGAACTGTGCCGTCTAAGGTGGGTGGTATGTAGTATGAACCAAATA11.8518518518518516No Hit
GCCTGCACACTCACTAGCGCGTGGCATACATGGTATCAGAGACAAAAGCA11.8518518518518516No Hit
TAACAAGTATGACAATTAGGAAGTTAGATTGGTCTTTGAGTTGTCTTCAG11.8518518518518516No Hit
ACATATATTTGTTAAAATCTAATAACTGATATTAACCATCTAACTATACT11.8518518518518516No Hit
CCCCAATGGGCCATGCTTCTGATTTATTCTCAAAAAACTCTTTGGTCATG11.8518518518518516No Hit
AACATAAGATTAAAATACATTATAAATTGAAACTCCATATTATTTTGCTA11.8518518518518516No Hit
GCCACGTTTATTGCATCTTATGCAGGGTCCAGGGTCTAGAGAAAGAAGGG11.8518518518518516No Hit
GATCAGAAACTTTTGATCTGAGCACTCTCCTAGACTCTCAATCAGAAGCT11.8518518518518516No Hit
CTGTAACATTGCATTTCAAGCAATATGAATTCAACTCTCCCCCAAACAAC11.8518518518518516No Hit
GGCCTGAAAGCGCTCCAAATGTCCACATCCAGATACTACAAAAAGAGTGT11.8518518518518516No Hit
CAATTCCTCAATAGTCATGTCAGTTATGTATCGCGAAGCAGGTGTGGAGA11.8518518518518516No Hit
CCATTCTGGGGTCTGGAGGATGGTGGCCCTCTTCTCACAGCCACCAATTA11.8518518518518516No Hit
GCTAGAAGATAACAGATCTAGGATACAAACACAGCTCCAACGCCCACTCA11.8518518518518516No Hit
ATCCCAATGATATTCGCGGCAACAACAAGCGGGACAATTGAATCGTTGCA11.8518518518518516No Hit
CTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGAC11.8518518518518516No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)