FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_E04_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_E04_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939266
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG79620.41056770963859524No Hit
GAAGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATT69530.35853771478487223No Hit
AGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTCT67940.3503387364085174No Hit
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC63430.3270825147246432No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA54350.28026067594646636No Hit
AAGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC53480.27577444249525335No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG52910.2728351860961828No Hit
AAGCAGAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC51220.2641205487024472No Hit
AAGCAGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGC45960.236996884388217No Hit
GCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTCTG41720.21513294205127093No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA41220.21255464696436693No Hit
GTCATCGAACAAGTCAAGGCCGCTGGAGCACTGCTTACTGAGGGGGAGGC39150.20188050530458432No Hit
AGCACGCACTTCCTCCTGGGCTAGGGGTTGTCAGTGAGGTTTCAACATTT38250.1972395741481571No Hit
GTGTAGGGACGTGCATGACCTCTGAGCAAGGCAGAGGGTATTGAAGAAAG36260.1869779597022791No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT35640.18378087379451813No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA32550.16784701015745132No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA32100.1655265445792377No Hit
AGCACGCACTTGGCTATTTGGGCCTTGGTATAGCATTGGGTCCTCCAAGC31680.16336077670623836No Hit
GTAGTAACAAGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGA31010.15990586128978695No Hit
CCTATGTGCTAGGCACACTTTCTAGGTGCTGAGTGGCCTTGGGCAGGTCC30970.15969959768283465No Hit
AGCACGCACTTGCTGTGAGTCACAAGACACTGATGTGTGTTTGCTTGCTT29530.15227410783255108No Hit
AGCACGCACTTGGGCACAACACAGACAAAGGTGGGGGGTCAGTGCCTGGG29360.15139748750300372No Hit
GCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCGTGGAGA28470.14680812224831455No Hit
ACATATATGAATCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCC27990.1443329589648867No Hit
CTCTTGGACAGGGGAATCAAAAGCTGCTTAGAGTGGTCAGGAGACGTGCC26520.1367527714093889No Hit
AGCAGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCG26350.13587615107984155No Hit
GTGTAGTGCAGTGTCAGTAGTGTGGGCTTGAGGCCTGGCTTTTCACCCGT25330.13061642910255736No Hit
GTGTCAGTAGTGTGGGCTTGAGGCCTGGCTTTTCACCCGTTCTCTCATTC23510.12123143498622675No Hit
CGTGTAGGGACGTGCATGACCTCTGAGCAAGGCAGAGGGTATTGAAGAAA23410.12071577596884595No Hit
GCTCAGAGGTGGGCGTGGAGATGGCGTAGCCATTGTTCCGGCAGAAGAAG22960.11839531039063234No Hit
CCCTTATAGCAACATGAGTTTAGAGCAAGAATCACACCCCGACTTTACAG22870.1179312172749896No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC22650.11679676743675184No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA22570.11638424022284721No Hit
GCTATAAGGGTTACGAGAGCTCATGCCAGGGAAGCACCTGGTACACAGTA22490.11597171300894256No Hit
ACCCTATCTCTTTGAGTCTCTTTATGGATCCTGTAAATAGGAACAGGGGG22060.11375437923420512No Hit
GCCCAAGGCCACTCAGCACCTAGAAAGTGTGCCTAGCACATAGGAGGCAC21680.11179487496815806No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT21610.11143391365599148No Hit
GTTATAAATCTGTGAGTGATTTCATAACCAAACCTCGTGCCTTGTAACTG21500.11086668873687261No Hit
ATACTGGACTCTTCAGCCTGAGCAGTTGGAAGGGTGGAGTTGCTTTCAGC21430.11050572742470605No Hit
CTTTCTAGGTGCTGAGTGGCCTTGGGCAGGTCCCTTACTTCTCTGAGCCT21130.10895875037256364No Hit
AAGCGGAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC21130.10895875037256364No Hit
CTACTGACACTGCACTACACCACCTCTCAGGAGAGAAGGCAAATATTTCC20760.10705081200825467No Hit
GTTCAATGACAGCATCCACTATACGGCTGGCAGCCTCCTCTATCAAGTCT20760.10705081200825467No Hit
GAAGCGGAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATT20410.10524600544742187No Hit
GCCTTGGGCAGGTCCCTTACTTCTCTGAGCCTCAGTTTCCATCTCTGTAA19410.10008941527361384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)