FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_E04_FluA_Rep1_Plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_E04_FluA_Rep1_Plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT21.9047619047619049No Hit
CCGTTCGATCTAAAGACTTCTATGGTGTTGGCTAATGCAGTTGCTGCCGG10.9523809523809524No Hit
ATTCTATTAACCATGAAGATTATCATTGCTTTGAGCTACATTCTATGTCT10.9523809523809524No Hit
GAAGGGATCCCATTATATGATGCGATCAAGTGCATAAAAACATTCTTTGG10.9523809523809524No Hit
GCAATAAACATGGCGACAGTCATGTTAAAGACAGAGAATACATGGTGTAA10.9523809523809524No Hit
ATCCGGAATCTTCACATCCCTGAAGTCTGCTTAAAGTGGGAGCTGATGGA10.9523809523809524No Hit
CATCCTGGACCCAGCAAACTGGTTTCTGAGTCAAAAAAGATAATTAAAAA10.9523809523809524No Hit
GTATTATTACTCTTGAGCTCTGGTTGGGATCTCTGTGCATGTCCTCATTG10.9523809523809524No Hit
CAACAGCACGGCAACGCTGTGCCTTGGGCACCATGCAGTGCCAAACGGAA10.9523809523809524No Hit
TGACTGTCGTCAGCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTC10.9523809523809524No Hit
GTATTGACCAGAACAGATTCAGGACCGTTAATCTCCCACATCATCGACGA10.9523809523809524No Hit
GTGTTTCCTCCACTCCTGGTCCTTATGGCCCAGTACCCGCTTCTCAGTTC10.9523809523809524No Hit
CTATTCAGATTCCCATTGATTTGATCGATTGCTGCTTGAGTGCTTTTGAG10.9523809523809524No Hit
CCGTAGAAGGGCCCCTTTTAAGGCCGTGTTTGATGATTAGATAGACGCAT10.9523809523809524No Hit
CTATTTCTAGTAGGTCCTAAAATATAAACTATTTACGTCAACCTAATAAA10.9523809523809524No Hit
CACCAAGAGGGATTTCCTGATAATTTTACAATCCTCAGACACCCTCATAT10.9523809523809524No Hit
TTGCAAGGTTGCTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCTG10.9523809523809524No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.9523809523809524No Hit
CAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGAGGC10.9523809523809524No Hit
CATGAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAAAATATGGCAC10.9523809523809524No Hit
GACCCTGCCACTGAGTGACCAGTTGCCTTGGGCAGGTCTCTCCCTGGCTT10.9523809523809524No Hit
CATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGTCCAC10.9523809523809524No Hit
GTCAACTATGACTTGCCTATTTATCTGCAATTTGGACTTAGGGTTGGACC10.9523809523809524No Hit
TTTCAAGGTAATATATGTGGACTTCTCTCCTTGTCACTCCAATTTCGATG10.9523809523809524No Hit
CCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTTCAAACAG10.9523809523809524No Hit
GTGTTGAGTGTTTGCGGATTTTCCAAGTGCACAGTGCAAGCTGTCGGTGG10.9523809523809524No Hit
AATCAACTCCACATAAAAGCACCGATTGATGCAGCTTTTGCCTTCAACAG10.9523809523809524No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC10.9523809523809524No Hit
TCATCGGTTTGATCAATGGTATACACACCATCTCCTCAACTATGATTCAC10.9523809523809524No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA10.9523809523809524No Hit
GCATTGCATGATCAAGGAAGTTAGGCGCGATCTGAACTTAGTCAATAGGG10.9523809523809524No Hit
GTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACAAAGAAGAAAT10.9523809523809524No Hit
GGCCACAAAGGCAGACTACACTCTTGACGAGGAAAGCAGGGCTAGGATTA10.9523809523809524No Hit
GTCGTACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA10.9523809523809524No Hit
GTTGTAACATTCAGTCAACGAGTTCATGAGGCACACAGCCCTGAGCCGCA10.9523809523809524No Hit
GGGCAAAGGCAAGTGCTCAGACTCAGACTCAGGGATTGTTGGACATGGAC10.9523809523809524No Hit
GCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATTGG10.9523809523809524No Hit
GTGTTGTGCCTGCGAGTGCACAGAAGTCAACAATTGAAGTTTGGGAACCT10.9523809523809524No Hit
CATTCTTCAAATTCCTTGTGTGCATCCACAATTGGCCATGCTTCTTATTT10.9523809523809524No Hit
ATGTTGAACATGCCCATCATCATTCCAGGGCTCAATGATGCTGTGCCATC10.9523809523809524No Hit
ACAGAACACACCAATATTCAGAGAGGGGGAAATGGGTGACAAATACAGAA10.9523809523809524No Hit
AAAATCCTCACCCAAACGATTATCATAGAAAACAAAATTCTCCACCAACT10.9523809523809524No Hit
TCTCTCACTTGATCCACCATTGCTCTTTGTGCAGCTGTTTGAAATTTTCC10.9523809523809524No Hit
CTAAGAGCCTGAACAACAAGGAGCAGTTTTCTTGACTCCGCTGAAAATCC10.9523809523809524No Hit
GGCTGATAAGAAGGAACCAGATAAGAGGCTGTTTTTGATCTGGGACAGAG10.9523809523809524No Hit
TTGAAGACCCAAGTCACGAAGGAGAAGGGATCCCATTATATGATGCGATC10.9523809523809524No Hit
TTAATGTAGGATTTGCTGAGCTTTTGGTATGAATTTCCTTTTTTAACTAT10.9523809523809524No Hit
GTTCAAGCTTTTCACAAATGCTTCTAGCTAAAGTTTCAACGAAGTACACG10.9523809523809524No Hit
ACAAATAATGAATCTAAAGTTGTGGGACAGGCGAGGTGACTCACGCCAGC10.9523809523809524No Hit
TTTTCAAGAAGGTCATCTTTCAGACCAGCACTGGAGCTAGGATGAGTCCC10.9523809523809524No Hit
CTTGCCCTAAACTTACCAATGGAAACGGAATATTCGCAACAATAGATATG10.9523809523809524No Hit
GCTTATGAGAGAATGTGCAACATTCTTAAAGGAAAATTTCAAACAGCTGC10.9523809523809524No Hit
GACTTAGCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCAC10.9523809523809524No Hit
GCAAAAGCAGGGCCCAGCTCAGTAGAGAATCTTGCTGCTGCTCCTTCCTG10.9523809523809524No Hit
TGCTTATGCATACTCAAAATCTAATTGGCTATCAAAGCTTCGCATTGCCC10.9523809523809524No Hit
CAGGAAGGTCACCTGAATTCGGGAGTTCGAGACCAGTCTGGCCAACATGG10.9523809523809524No Hit
CTCTATCAATGTCTGTAAACATAGGAAAATTATATATTATGATAGCCCCA10.9523809523809524No Hit
CACCTGTACCATGAGGTCCCGGAGGGCAGGGGCTGAGGGTCATCCCTCTC10.9523809523809524No Hit
TCTTTGTACAGAAAACGTAGGTTGCACACTGGTTTGGCCTGCGGAGGCAC10.9523809523809524No Hit
TCCTCTTGCTGTCGGGCATTCACAAAACATAAACTAAAGCATACAAACAA10.9523809523809524No Hit
GTTAGTAATCTTGTCAATGGCATTTTGTGTGCTCTTAAGGTCGGCTGCAT10.9523809523809524No Hit
ATGCCAAACAATGAACAATTTGACAAATTGTACATTTGGGGGGTTCACCA10.9523809523809524No Hit
AGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAA10.9523809523809524No Hit
ATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCAGCTGGAACAGATAT10.9523809523809524No Hit
AGAGGGATATTCCTTGGTGGGAATAGACCCTTTCAAACTACTTCAAAATA10.9523809523809524No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCCCTTTTAAGGCCGTG10.9523809523809524No Hit
GCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTC10.9523809523809524No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT10.9523809523809524No Hit
ACAATGGTGAGGATGCTACATCTGGTCTAACTCACATAATGATTTGGCAT10.9523809523809524No Hit
CTTTGTACAGAAAACGTAGGTTGCACACTGGTTTGGCCTGCGGAGGCCCT10.9523809523809524No Hit
CTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA10.9523809523809524No Hit
CAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTG10.9523809523809524No Hit
ACAGAGCTCAGTAGGAAAAAGAGGCGCACTATTTGCAGGTCGGAATGAGT10.9523809523809524No Hit
CCAAAAAAGAATGGTAAAAAGAAGAATGGAAATGTTGGTATATGTAGAGA10.9523809523809524No Hit
CATATCTCCTATCTCAAGGACACAGTATTTCTCCCATTTATGTGGCTCAA10.9523809523809524No Hit
TAAGTGTGTAGTCTGGTTCGTCTTAGCGACAAAAGTTAGAAAACCACTTT10.9523809523809524No Hit
ATTCCCACCAAGGAATATCCCTCTTTTTCGAAGTCGTACCCACTGGATAC10.9523809523809524No Hit
AGCCTGTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTT10.9523809523809524No Hit
CTAGAGATATTTGGTGAATAGGTAAGCCTGGAGCTTCCATTATACTCATG10.9523809523809524No Hit
CATGGAAGCAAGTACTGTCAGAATTGCAGGACATTGAAAATGAGGAGAAG10.9523809523809524No Hit
CCACAGAAGCTGCTTTTGGTCTAGTGTGTGCCACTTGTGAACAGATTGCT10.9523809523809524No Hit
GCCCAGGTGCTGGGGCGCCCTAACCACCCACACGGTGCTGGGCCGTCAGG10.9523809523809524No Hit
ACACAGTATTTCTCCCATTTATGTGGCTCAAGTCTCGGGTCAGTGAGAGA10.9523809523809524No Hit
TGATAACACTTGTCAGGATCGCATGACACATAAGGTTCTCTTGTCACCCA10.9523809523809524No Hit
CTGTTAACTATGACTTGCCTATTTATCTGCAATTTGGACTTAGGGTTGGA10.9523809523809524No Hit
CTTAGTAACATATCTCCTATCTCAAGGACACAGTATTTCTCCCATTTATG10.9523809523809524No Hit
GGCTCCAATAGGCCCATCGTAGATATAAACATAAAGGATCATAGCATTGT10.9523809523809524No Hit
ATTTTGAAGTAGTTTGAAAGGGTCTATTCCCACCAAGGAATATCCCTCTT10.9523809523809524No Hit
GATAAAGCACTTCCTTCTGCCCACGTGCTAGTTGGGAGTGGGGAGAAAGG10.9523809523809524No Hit
CAAAGCGTCATTACTTATGAAAACAACACTTGGGTAAATCAGACATATGT10.9523809523809524No Hit
GACCAAGCCCGGGGAAAGGCGGATGTCGGCAGGGCTGGAGCTGAGCAGCC10.9523809523809524No Hit
CTCATGATTTGGTGCATTCACTATGAGAGCAAAATCGTCGGAGGATTGGA10.9523809523809524No Hit
CATCATAAGGGTAACAGTTGCTGTGGGCTTTGCTTCGTTCAACAAAAAGG10.9523809523809524No Hit
GACTAAGGGGATTTTGGGGTTTGTGTTCACGCTCACCGTGCCAAGTGAGC10.9523809523809524No Hit
GGAATGGGGACCCGAACAACATGGATAGAGCACTTAAACTATACAAGAAG10.9523809523809524No Hit
AGCGAAAGCAGGCCTGCCCCCTCGTGGGGTGGGCTTTTTTTTTTTTTTTT10.9523809523809524No Hit
GTTAAAGCACTTCCTTCTGCCCACGTGCTAGTTGGCAGTGGGGAGAAAGC10.9523809523809524No Hit
CACTAAAGAAGAGCCCTTGTCAAGGTTACCAGCAACCTCCACGTTGCAAA10.9523809523809524No Hit
GCAGCAACTGCATTAGCCAACACCATAGAAGTCTTTAGATCGAACGGATT10.9523809523809524No Hit
TGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCAGCCGGAACA10.9523809523809524No Hit
GGAATGGAGACCCAAATAATATGGACAAAGCAGTTAAACTGTATAGGAAA10.9523809523809524No Hit
TTCCCACGTTGGTCTCGAACTCTCAAAAACCGATCAATGCTAACCACCAC10.9523809523809524No Hit
GAGCTAGGATTCATCCACAGCTCCGACGCCACCGACGGTCTAATTCTAGA10.9523809523809524No Hit
GGGCATGGCGCCGGTGCAGAAGGGAAGGAGGTCGGGGAAAGAAGATGCAG10.9523809523809524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)