FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_E03_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_E03_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156783
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTAGAA99230.4600833741734796No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT90430.419281865630432No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT69160.32066276486786105No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC66510.3083759469543297No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA65950.30577948731977206No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT61180.2836632150754156No Hit
TTTTTAAACTATTCAGCATTAATTGATGGCCATCCGAATTCTTTTGGTCG60610.2810203900902409No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC45860.21263149793001893No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC45010.20869044312756546No Hit
GATCTTATGATCTCAGAAAACTCTTCCTTCTTAATCCTTCCAGACTCGAA44920.20827315497201157No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA43750.2028484089498109No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA41430.19209164760664377No Hit
CTATAAACACAAGCCAAAGGGGAATTCTTGGACACAGTCAACAGAACACA40380.18722328579184833No Hit
GCATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG38570.17883115733015328No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC35960.16672980081909028No Hit
ATTCAGCATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT34670.16074867058948444No Hit
GATCTTCATGATCTCAGAAAACTCTTCCTTCTTAATCCTTCCAGACTCGA33780.15662215438456256No Hit
GCCATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA32280.1496673517919976No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG31320.14521627813275606No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA30520.1415070500833881No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGCATTAATTGATGGCCATCC30300.1404870123698119No Hit
GTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTC27880.12926659752047379No Hit
GCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTAGAACAGATC26640.12351729404395342No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGTTC25540.11841710547607247No Hit
ACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAGTCATGTTAAG24920.11554245373781229No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCAGTCTCGCACT24640.1142442239205335No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA24500.1135951090118941No Hit
GCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTC23910.11085955332548522No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA23750.11011770771561163No Hit
CTATGGACACAGTCAACAGAACACACCAATATTCAGAGAGGGGGAAGTGG23580.10932949675512094No Hit
GTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCCTAGCT23550.10919040070326963No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC23520.10905130465141834No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA23300.10803126693784215No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC23290.10798490158722504No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTTTGCCG23030.10677940247118047No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT22950.10640847966624366No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA22220.10302380907119539No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA21600.10014915733293521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)