FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_E03_FluA_Rep1_Plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_E03_FluA_Rep1_Plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGA12.631578947368421No Hit
CAGACTAGGCAGATGGTACATGCAATGAGAACTATTGGGACTCATCCTAG12.631578947368421No Hit
TGCTGTGGACTGGGGTGCAGGGTCAGGCATGGGAGTCATCATCCACATCA12.631578947368421No Hit
GTTCTCACACTGCCTTATCATAATAATCGCCTACCCAACAACACCCACAC12.631578947368421No Hit
AGATACACCAGTCCACGATTGCAATACAACTTGTCAGACACCCAAGGGTG12.631578947368421No Hit
CAAAACGCCACTCTCTACTTGACACCTGACACAAAACCTTCCCACAATAC12.631578947368421No Hit
GACCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC12.631578947368421No Hit
ACTTTGTTTAAAAAATAAAAGCCAAATACAACCAACTCAAATCAATCGAC12.631578947368421No Hit
ATTGAAACAAGTTATAATAAATTATTAATATATAATAATCGAAAAAATAG12.631578947368421No Hit
ATGCAAACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTG12.631578947368421No Hit
ATATGATAGTGAAGAACCTGAATTAAGGTCACTTTCAAGCTGGATACAGA12.631578947368421No Hit
CTGCGTGCCTCTCCGGCTTGCAGGCTGGCACCTGTGCACAAACCGAGCGT12.631578947368421No Hit
CTCTTCACTAAGAAAGCGCTGCTCTGGAGGTTTAGAGGGGTGTAGCATCC12.631578947368421No Hit
TTGTCATTTAGCAAGGCCCCTTGAGTCAAGAAGAAGGTTCTGCATTCCAA12.631578947368421No Hit
TAATACTGTTAGTTAATGTTTAATTTGGGGCTAATATAGACTAGGTGTTT12.631578947368421No Hit
CCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAAGATGTCTT12.631578947368421No Hit
GTTTAACTCATAAGGGTTAAATAAACTTGGTCAAGATCAGGTTCAAGGAT12.631578947368421No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC12.631578947368421No Hit
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA12.631578947368421No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.631578947368421No Hit
GTGGGAAAGTGGACAGACATCAGGGCAAGATGCTTGGCCAGGGTCAGGGA12.631578947368421No Hit
GCCTGGAGAGTCATCTGCCCTGGGCTTCTGCATCCTGGTGTGTGCAGTGC12.631578947368421No Hit
ACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGA12.631578947368421No Hit
GATGATGATTGCCCAAATGAAATACTATAAAGAGACAAGAAAAACAAAGA12.631578947368421No Hit
GCTTCAACCCGATGATTGTCGAACTTGCAGAAAAAGCAATGAAAGAGTAT12.631578947368421No Hit
ATCCTGGTCTGGCCCCCCCTAGCAAGTGAGTGGCGTCCATGTCTGGGCTC12.631578947368421No Hit
AGTGTGCGCCTGTCGTCCCAGCTACTCAAACCCAGATGTTTGAACTAATG12.631578947368421No Hit
ACTCAACTCAGTCCATTCAGCCAAGCGTATTACTCCTCAAACTAGAAATA12.631578947368421No Hit
CGTTCATCGTCATCACCTAAATAAAGGGTCGCTATCTGCAACTACAAACC12.631578947368421No Hit
GGGTACTCACTCCCAAGCCTCTCTGCTTCCAGCCTGTTATCCAAGCCTAC12.631578947368421No Hit
AGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAA12.631578947368421No Hit
TAAGAAAGCCCCTCAATTACTATGTGGTCACTCAATACATCCATGTTGAC12.631578947368421No Hit
TTGAATAGACGGGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGC12.631578947368421No Hit
CAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCATAT12.631578947368421No Hit
CTTTTGAGCTGAGCACTCTCCTTGACTGGCACTCAGAAGCTCTGGTCCCT12.631578947368421No Hit
AACTGATGCAGTGCTAATAATCGCCACAATAGGAAATAGGCCTCAGAGCC12.631578947368421No Hit
CAATAGTCCTGTCCTTGATTGGGTTTGACCCCACAGCTTCTTTATCTCGA12.631578947368421No Hit
ACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTCGCAAG12.631578947368421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)