FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_E03_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_E03_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575108
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT57450.36473689423201455No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTAGAA48860.31020095129984737No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC43670.2772508297843704No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT36900.2342696500811373No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA33730.21414404599557618No Hit
TTTTTAAACTATTCAGCATTAATTGATGGCCATCCGAATTCTTTTGGTCG31800.20189091795610206No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT31790.20182743024605299No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC30600.1942723927502114No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC26540.16849638247028143No Hit
GATCTTATGATCTCAGAAAACTCTTCCTTCTTAATCCTTCCAGACTCGAA26410.16767104223964327No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC25530.16208412375532344No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA24100.15300538121830376No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC22660.143863150971235No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA21400.1358636995050498No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC20740.13167351064180996No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG20360.1292609776599446No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA19210.12195989100429938No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA19200.1218964032942503No Hit
GCATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG18920.12011874741287581No Hit
GTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTC18700.11872201779179586No Hit
GTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCCTAGCT18640.11834109153150134No Hit
CTATAAACACAAGCCAAAGGGGAATTCTTGGACACAGTCAACAGAACACA18270.11599204625968504No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG16970.10773864395330353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)