FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_D07_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_D07_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659418
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG49780.2999846934286599No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA43150.260030926505558No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT42390.25545100752191435No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG32010.19289895614004426No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG27870.16795045009756432No Hit
TCTCTGTACACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCA27660.16668494616787333No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA25670.15469278988175372No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT24940.1502936571737802No Hit
CAATAGGATGGGGGCTGTAACCACCGAAGTGGCATTTGGCCTGGTATGTG24490.14758186303872803No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA23650.142519847319964No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA23240.14004910155247202No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG22190.13372158190401695No Hit
GAGTAAGGAATATCCCTAGCAGAGTAAGCATCTATTGGACAATAGTAAAA20920.12606829623398083No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG20810.1254054132231903No Hit
CTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA20740.12498357857995995No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG20290.12227178444490779No Hit
GTACGTGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT19750.1190176314828452No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGAATGAGA19720.11883684520717505No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG19600.11811370010449446No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC19400.11690845826669351No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA19260.11606478898023283No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT19180.11558269224511243No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC18860.11365430530463089No Hit
CTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC18650.1123888013749399No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA18380.11076172489390859No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATGCTGTTTGAGGT18250.10997831769933795No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT17730.10684468892105546No Hit
CTCATAAGCACCTCTTGTTTTCCGCCCATTCTCACCTCTCCAAAAATTTC17700.1066639026453853No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC17120.1031687013157625No Hit
GTCCTGAATTCTCCCTTCGACTTCAGAGAATTCTTTTTCAATCTGATGGA16700.10063769345638049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)