FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_D04_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_D04_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588756
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG73100.46010841186437695No Hit
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC57480.36179249677105857No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA46660.2936888987358663No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG45120.28399578034638423No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA34770.21885047168980007No Hit
AGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTCT34260.2156404130023742No Hit
GAAGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATT34160.2150109897303299No Hit
GTAGTAACAAGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGA32780.20632494857611866No Hit
AGCACGCACTTGGCTATTTGGGCCTTGGTATAGCATTGGGTCCTCCAAGC31630.19908658094760934No Hit
GTCAATGAGGTTTCAACATTTTGTTAGCTGTGTGCAGCATTCCCAGAGCT30430.19153350168307784No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT30120.18958228953974052No Hit
ACATATATGAATCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCC30120.18958228953974052No Hit
AAGCAGAGCTGTCAGAGTAGAGTCAATGTCGTAGGGTGAAGGTATGGGCA29950.18851226997726522No Hit
AAGCAGAGCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGC26320.16566420520205746No Hit
AAGCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC26310.16560126287485302No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA26150.16459418563958217No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA24230.1525092588163318No Hit
AAGCAGAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTC22380.14086492828351238No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC21990.13841017752253965No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA21810.13727721563285994No Hit
AGCACGCACTTTGGTTTTTAGAATTTTCAGCTGTTCTGCTCTGGTTTCTC21080.13268242574693662No Hit
GCACAGCTGGGTGTGGGGTCCTCAGGCCTAGGGCGGAACAGCCTATTCTG20150.12682878931692468No Hit
GTGTAGGGACGTGCATGACCTCTGAGCAAGGCAGAGGGTATTGAAGAAAG19940.12550700044563168No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC19930.12544405811842724No Hit
GCACAGCAGGTTGTCAAGTGTTGGGGTTGGGGGCCTTATCCAGCCACTCC19400.1221081147765925No Hit
AGCACGCACTTGCTGTGAGTCACAAGACACTGATGTGTGTTTGCTTGCTT18610.1171356709274426No Hit
AGCACGCACTTGGGCACAACACAGACAAAGGTGGGGGGTCAGTGCCTGGG18370.11562505507453631No Hit
AGCACGCACTTCCTCCTGGGCTAGGGGTTGTCAATGAGGTTTCAACATTT17590.11071555355259084No Hit
CCTATGTGCTAGGCACACTTTCTAGGTGCTGAGTGGCCTTGGGCAGGTCC17360.10926788002688897No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT16930.1065613599570985No Hit
AGCACGCACTTCCTCCTGGGCTAGGGGTTGTCAGTGAGGTTTCAACATTT16630.10467309014096564No Hit
GCCCATACCAATGCCATCGCCGCGATACTGCTCAGAGGTGGGCGTGGAGA16560.10423249385053464No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG16500.10385483988730806No Hit
GCTCAGAGGTGGGCGTGGAGATGGCGTAGCCATTGTTCCGGCAGAAGAAG16470.10366601290569477No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT16230.10215539705278848No Hit
GTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGACGTGAAAGCTGAAGA15970.1005188965454733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)