FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_D03_FluA_Rep1_Plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_D03_FluA_Rep1_Plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995917
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCT125830.6304370372114673No Hit
GTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATC116110.5817376173458115No Hit
GTCCTGGAGGGATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCA105270.5274267416931666No Hit
TGGTATGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTG101990.5109931926026984No Hit
CTCACACCATTTCATAATTCCCCATCTCCAGTGTTTATGGTCTTACAGGT93020.4660514440229729No Hit
GGTATGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGT91690.45938784027592333No Hit
GATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACGA91330.45758415805867675No Hit
TCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAATGACTCCCACTTG72000.3607364434493018No Hit
ATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTGCTGAGCAAGGCAGG68430.34284992812827386No Hit
GTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTGAAGAAAGACAGTTT66020.3307752777294848No Hit
CTCCAGGACAGTCATCCACCTCAGGTAGAAAGTTTCTGCCCTTTCCCCAC58140.2912946780853112No Hit
CCAGACCCTCAAGAGCTTACATTCTAATGAGAGGGAGACACGTACTGAAC51450.25777625021481354No Hit
ATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCATGCTTAACACC50930.25517093145656855No Hit
GAGCAAAAGCAGGGAACAAAGGGACAGAAACCAGAGCCTCAAGAGCTTAC50830.2546699086184445No Hit
GATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAAAA50460.2528161241173857No Hit
GAGCAAAAGCAGGGAACAAAGGGACAGAAACCAGACCCTCAAGAGCTTAC48000.2404909622995345No Hit
GCAAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTGC48000.2404909622995345No Hit
GTTTTAAGTACCGCTGATTTTCTTGCAGCTATTTGCCTAATTCCTCTGAC47140.23618216589166782No Hit
GAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGT46550.23322613114673604No Hit
GATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAG45990.23042040325324148No Hit
GGTGTGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGT45520.22806559591405853No Hit
CTTAAAGACAGTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGG45310.22701344795399808No Hit
GATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAG45100.22596129999393763No Hit
GCCTAGTAGTAGGAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCCA44810.22450833376337792No Hit
TTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATCACCAGCACCAATT43740.21914738939545084No Hit
GTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTGATTATACTGT42320.21203286509408956No Hit
ATTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTG42140.21113102398546635No Hit
TGATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCA41970.21027928516065547No Hit
GACCCTCAAGAGCTTACATTCTAATGAGAGGGAGACACGTACTGAACAAG41550.20817498924053457No Hit
GTCTAAGCTCACACAGCCAGGTGGAGAGGAGCAGAGCTGAGATTCAAGCC41090.205870284185164No Hit
ATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGG40150.20116066950679815No Hit
CTTCTGTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTGATTAT39260.19670156624749424No Hit
TTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTGA38410.19244287212344No Hit
GGCTAAGACAGGAACAGGCAGGACACAATTGTTACTTGACGGCCCTGCCT38100.1908897013252555No Hit
GGATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGA38040.19058908762238108No Hit
CCTATGAATCCCTCCAGGACAGTCATCCACCTCAGGTAGAAAGTTTCTGC37730.1890359168241966No Hit
TCATATCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTGCC37240.18658090491738885No Hit
AGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTC37150.18612998436307723No Hit
GCATTAGGCTGAGGGATGGGCCTAGTAGTAGGAATAAAAGAGGGATAAGA36920.18497763183539195No Hit
CCACAGAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAG36590.18332425646958264No Hit
GTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGGTAATTACAGA36160.18116985826564933No Hit
GGCCACAAGGTGGGAAGTAGGAGATAATGACTCCCACTTGTGTATTGATA35700.1788651532102788No Hit
GTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTCC35620.17846433493977956No Hit
GAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAA34160.1711494015031687No Hit
ACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACT33520.1679428553391749No Hit
ACTTAATATTATTCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAAT33460.1676422416363005No Hit
GTTTATCACCAGCACCAATTCACAAGGATGAGCCTGAGGCTCAGAATGGT32850.16458600232374393No Hit
ACTCACACCATTTCATAATTCCCCATCTCCAGTGTTTATGGTCTTACAGG32780.16423528633705708No Hit
GTGTATTGATAGGATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGT32680.16373426349893305No Hit
CTTGTGTATTGATAGGATAAATACCTTACTTAGTTCAGTTTATACAGCCT32440.16253180868743541No Hit
TGGTGTGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTG32340.16203078584931138No Hit
GGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGA32040.16052771733493928No Hit
TTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGCTAGTT31910.15987638764537804No Hit
GTGGATGACTGTCCTGGAGGGATTCATAGGAGAGGCACATTTCAGGCCAA30930.15496636383176254No Hit
CTTCATATCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTG30260.15160951081633153No Hit
GCTCCATACCCAGCACATAAACTTAATATTATTCCCCACACAGGCCACAA30210.15135899939726952No Hit
ACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAG30000.15030685143720907No Hit
GTATAAACTGAACTAAGTAAGGTATTTATCCTATCAATACACAAGTGGGA29420.1474009189760897No Hit
CCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGGTGTGAGT29010.14534672533978116No Hit
ATATTATTCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAATGACTC28350.14203997460816256No Hit
CCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGGTATGAGT28090.1407373152290401No Hit
TTTCAGGCCAAGAGAGTTTCATGCTTAACACCACAGAGATATGAAGATCA27930.13993567868804163No Hit
GTATTTATCCTATCAATACACAAGTGGGAGTCATTATCTCCTACTTCCCA27440.13748066678123388No Hit
GTGTTTATCACCAGCACCAATTCACAAGGATGAGCCTGAGGCTCAGAATG27380.13718005307835945No Hit
CTCATACCATTTCATAATTCCCCATCTCCAGTGTTTATGGTCTTACAGGT26980.13517596172586335No Hit
GTTTATGGTCTTACAGGTGAATGCTTTGTCGTCTTCACCACCAGAACATC26930.13492545030680134No Hit
GAATGGTGAAGCACCTTGTCTAAGCTCACACAGCCAGGTGGAGAGGAGCA26870.1346248366039269No Hit
GTCTTACAGGTGAATGCTTTGTCGTCTTCACCACCAGAACATCTGCTTAT26470.1326207452514308No Hit
GTGGTAGTGTGTTCTCCACGTCATTTCTGTGACAATCAAATGAGATCACA26380.13216982469711916No Hit
TCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTGCCTCTCC26260.13156859729137035No Hit
GGATGAGCCTGAGGCTCAGAATGGTGAAGCACCTTGTCTAAGCTCACACA26250.1315184950075579No Hit
GTGAAGCACCTTGTCTAAGCTCACACAGCCAGGTGGAGAGGAGCAGAGCT26210.13131808587230834No Hit
CTTCTCTTCTGTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTG26130.13091726760180908No Hit
GCATTCACCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGG25930.12991522192556101No Hit
TCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAAAAGAGCATGAGT25920.12986511964174863No Hit
GTGCAGCCAAGGCAGGTAGAGCAAAAGAGCATGAGTGCTGAAGTCTGACA25580.12816164199212693No Hit
CTTAAGGGGAGGCAGGCATTAGGCTGAGGGATGGGCCTAGTAGTAGGAAT25520.1278610282892525No Hit
GCTTAACACCACAGAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAG25350.12700928946444165No Hit
GTGCTGAAGTCTGACAAACCAGGGCTTGAATCTCAGCTCTGCTCCTCTCC25160.126057346072006No Hit
TCACCATTCTGAGCCTCAGGCTCATCCTTGTGAATTGGTGCTGGTGATAA25140.1259571415043812No Hit
CTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATCAC24580.12315141361088663No Hit
CACTTAAGGGGAGGCAGGCATTAGGCTGAGGGATGGGCCTAGTAGTAGGA24320.12184875423176414No Hit
ATTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGCTAGT23460.11753995782389749No Hit
CAATTGTTACTTGACGGCCCTGCCTCTTTGGGCATAAAGCCCCGCACCTC23160.1160368893095254No Hit
GGCCAAGAGAGTTTCATGCTTAACACCACAGAGATATGAAGATCACTGAG22860.1145338207951533No Hit
GAGCAAAAGCAGGAAGGTGGGCAAGCTATGCAGGTAACATTGGAGCTAAA22810.11428330937609128No Hit
AGCTTAGACAAGGTGCTTCACCATTCTGAGCCTCAGGCTCATCCTTGTGA22280.11162788833403393No Hit
AGCGAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTG22240.1114274791987843No Hit
CTTCAGCACTCATGCTCTTTTGCTCTACCTGCCTTGGCTGCACAGATGCC22210.1112771723473471No Hit
AACTAGCAACGTACTTAAAGACAGTATAATCACACCAAGCTCCTAGTAAA21980.11012481981966184No Hit
GCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTCCTTCCCCACC21760.10902256957578897No Hit
CAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCT21650.10847144445385254No Hit
ATTCACAAGGATGAGCCTGAGGCTCAGAATGGTGAAGCACCTTGTCTAAG21420.10731909192616727No Hit
GCAAAAGCAGGGAACAAAGGGACAGAAACCAGAGCCTCAAGAGCTTACAT21180.10611663711466959No Hit
GTGCTTCACCATTCTGAGCCTCAGGCTCATCCTTGTGAATTGGTGCTGGT21140.10591622797941999No Hit
TCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCATGCTTAACACCAC20860.1045133640326727No Hit
GAATACTACCAGGTGTTTATCACCAGCACCAATTCACAAGGATGAGCCTG20850.1044632617488603No Hit
GGGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTG20840.10441315946504791No Hit
CCTCTCCACCTGGCTGTGTGAGCTTAGACAAGGTGCTTCACCATTCTGAG20780.10411254576217348No Hit
GTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTCCTTCCCCACCACTTC20740.10391213662692388No Hit
TTGTGGTAGTGTGTTCTCCACGTCATTTCTGTGACAATCAAATGAGATCA20530.1028599886668634No Hit
ACCATAAACACTGGAGATGGGGAATTATGAAATGGTGTGAGTGGGGAAAG20520.102809886383051No Hit
GCAAAAGCAGGGAACAAAGGGACAGAAACCAGACCCTCAAGAGCTTACAT20450.10245917039636418No Hit
ATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAA20390.10215855669348976No Hit
AGCAAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTG20140.1009059995981797No Hit
GGATATTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGC20110.10075569274674248No Hit
GTACTGAACAAGTAATTTCGGTGTGCTAGTTTAGAAGTGGGTTCGTATAG20000.10020456762480603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)