FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_D01_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_D01_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262162
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATGTCC53850.42664887708550886No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG47210.3740407332814647No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT39090.3097066779066395No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA33190.2629614898879859No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT28610.22667454732435297No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG26210.2076595555879515No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT24950.19767668492634066No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT22610.17913706798334922No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA21210.16804498947044832No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA20440.16194434628835286No Hit
GGATTGGGTGATGCTCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC19010.15061458037874695No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA18000.1426124380230113No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT17730.14047325145266615No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT17600.13944327273361104No Hit
CCCATATATAGAAGAATAGACGGAAAGTGGATGAGAGAACTCATCCTTTA17520.138809439675731No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC17180.1361156491797408No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG17140.13579873265080075No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA16860.1335803169482206No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT16750.1327087964936355No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT16610.13159958864234544No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT16530.13096575558446538No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG16370.12969808946870529No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC15970.12652892417930503No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG15940.1262912367826No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA15840.12549894546024995No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC15630.12383513368331481No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC15480.12264669669978971No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC15250.12082442665838457No Hit
GTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGA15040.11916061488144945No Hit
GTGCTGGCTAGCACTACAGCAAAGGCTATGGAACAGATGGCTGGATCGAG14990.11876446922027442No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC14940.11836832355909938No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC14330.11353534649276399No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG14110.11179230558359386No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG14090.11163384731912385No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC14080.11155461818688885No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG14060.11139615992241884No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT13730.10878159855866362No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT13540.10727624504619851No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT13370.10592934979820341No Hit
GTACTATGTTCTTTTCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG13340.10569166240149838No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC13230.10482014194691332No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC13010.10307710103774317No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG12910.10228480971539312No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT12850.10180943492198308No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT12840.10173020578974808No Hit
CCTTTGGCATGTCCGCAAGCGATTTGCAGACAATGGATTGGGTGATGCTC12840.10173020578974808No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC12820.10157174752527805No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG12700.10062099793845798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)