FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_C08_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_C08_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2462086
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA81060.32923301623095214No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC66330.26940569906981315No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC66280.26920261924238226No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA57380.2330544099596846No Hit
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC52650.21384305828472278No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC51200.20795374328922708No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG44670.1814315178267534No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC43930.1784259363807763No Hit
CCACACTGGCAAACCCTAAGGCCCCTTGCCAGTGCTCACCTTGCGTGGCC43350.17607021038257803No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA39790.16161092666949894No Hit
CTCTCTCACCCCACCTTCCTCTAGCATGCACCTGGCTGCCTTTGCCTCCC39520.16051429560137218No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA39020.15848349732706332No Hit
ACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAGTCATGTTAAG35310.14341497413169157No Hit
ATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGT33920.13776935492911296No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT33220.13492623734508055No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA32090.13033663324514252No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA31910.12960554586639134No Hit
TCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGAGG31630.12846829883277838No Hit
CTTCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGA30580.12420362245672979No Hit
GCAGGCAGGATGTGAGGTTGCTCCCTCTCCACCTGCTTCAGCCCTGCCAC30510.12391931069832653No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA30360.12331007121603389No Hit
GTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCCTAGCT30160.12249775190631033No Hit
CGTCCTAATAACAAGGGACCAATACATGGGTACCACACTGGGTGCTGAGA29930.12156358470012825No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC29900.12144173680366975No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT29550.12002017801165353No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT29210.11863923518512351No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC28410.11538995794622933No Hit
CTTTCTCTAGACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAG27430.11140959332858397No Hit
GTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGTCCTCCTGT27010.10970372277816452No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA26530.10775415643482804No Hit
CCTTGCGTGGCCCATCTGCCGCTGCCCATGCCGCTGACGGCTTCTTCCTG26370.10710430098704918No Hit
CCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG26350.10702306905607684No Hit
CGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG25790.10474857498885093No Hit
GAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGTTTGA25380.10308332040391766No Hit
CCTCGTGTGGCCCATCTGCCGCTGCCCATGCCGCTGACGGCTTCTTCCTG25320.10283962461100059No Hit
GAGTAGGGGAGGGCCAGAGACAGAGAGGAGACAGCAGCTAGAGCACCCGT25320.10283962461100059No Hit
GTCCAGGGTCTAGAGAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGG25270.10263654478356972No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC25110.10198668933579086No Hit
TTGCATGCCCCTCTTCATTCCTCAGGGCTCTGTCCTCAAACCATCCTTTT24760.10056513054377467No Hit
ATCCACAAGTATCTTAACTGTTCACAAATTTAGCTTTCTTGTTTTTGAGA24730.10044328264731614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)