FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_C08_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_C08_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736983
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC59500.3425479696692484No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA56860.3273492026116548No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC46920.27012354179632153No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA37440.2155461509985993No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC33700.19401456433367512No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA33280.1915965786654216No Hit
CTCTCTCACCCCACCTTCCTCTAGCATGCACCTGGCTGCCTTTGCCTCCC30910.17795223096599103No Hit
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC29740.1712164137472848No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA29230.16828028829297695No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA27390.157687208222533No Hit
CCACACTGGCAAACCCTAAGGCCCCTTGCCAGTGCTCACCTTGCGTGGCC27250.1568812129997818No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA26040.1499151114317181No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC25170.14490642683319294No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA24220.13943717353595286No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT23630.13604047938293007No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA23590.13581019503357258No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG23270.13396792023871276No Hit
CGTCCTAATAACAAGGGACCAATACATGGGTACCACACTGGGTGCTGAGA22550.12982280195027815No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC21340.12285670038221445No Hit
ATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGT20930.12049628580130031No Hit
GTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCCTAGCT20810.11980543275322787No Hit
ACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAGTCATGTTAAG20620.11871158209377985No Hit
TCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGAGG20360.11721473382295623No Hit
GCAGGCAGGATGTGAGGTTGCTCCCTCTCCACCTGCTTCAGCCCTGCCAC20260.11663902294956255No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT19920.11468160598002398No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC19180.11042134551691063No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT18990.10932749485746261No Hit
CTTCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGA18810.10829121528535399No Hit
CCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG18240.1050096633070099No Hit
CTCTCCACCTGCTTCAGCCCTGCCACTTACCCCAGTGAGCCTCTGCCCTT17790.1024189643767383No Hit
CCTTGCGTGGCCCATCTGCCGCTGCCCATGCCGCTGACGGCTTCTTCCTG17770.10230382220205955No Hit
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA17630.10149782697930838No Hit
CTTTCTCTAGACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAG17430.10034640523252099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)