FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_C03_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_C03_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389651
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT50320.36210530557672393No Hit
AAGCAGAACTAGCAGAAAAATTACACTGTTGGTTCGGTGGGAAAGAATTT29220.21026862140206426No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG26390.18990379598906487No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT26080.18767302006043243No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA24300.17486404859925261No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT23760.17097818085260258No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT19940.14348926457074473No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC19740.1420500542942077No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT19200.13816418654755763No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG18740.13485400291152239No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCGGGCACTGAAG18690.13449420034238813No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGA18320.13183166133079455No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT17830.12830559615327877No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCTCCATTCTTTTGG17790.12801775409797136No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGTGATTTTTCTACCTCCAAA17540.12621874125230004No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA17440.12549913611403152No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT16260.11700779548246287No Hit
GTAGAAGTACCATACATTTGTGCAGAAGGAGAAGACCAAATTACTGTTTG15890.11434525647086931No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT15860.11412937492938874No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC15590.11218644105606371No Hit
GGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTTGGAATGGATAAAA15510.11161075694544889No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT15390.11074723077952667No Hit
GTCCTATTGTCCAGGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA15250.10973978358595073No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG15070.10844449433706736No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGT14970.10772488919879883No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATAATGACAAGC14920.10736508662966457No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA14850.10686136303287659No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG14820.10664548149139604No Hit
GTACAAACGCATCAAATGTTCAGGCTGTGAATCGTTCTGCAGCAAAAGGG14460.10405490299362935No Hit
GTCCAGGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG14290.10283157425857282No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG14290.10283157425857282No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA14160.10189608757882375No Hit
CCATAGAGGTTCTTCATTTGGGTTTTGTTATCTGAATGGAACCCCCAAAC14100.10146432449586262No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT13950.10038491678845984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)