FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_C03_FluA_Rep1_Plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_C03_FluA_Rep1_Plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1449518
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCT55800.38495555074169485No Hit
GTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATC48480.33445600537558No Hit
GTCCTGGAGGGATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCA46810.32293493423331066No Hit
GATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACGA43050.29699527705071616No Hit
TCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAATGACTCCCACTTG32370.22331561250015522No Hit
ATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTGCTGAGCAAGGCAGG30290.20896601490978378No Hit
CCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGGTATGAGT28490.19654809391811626No Hit
GTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTGAAGAAAGACAGTTT26950.18592387262524507No Hit
CTCCAGGACAGTCATCCACCTCAGGTAGAAAGTTTCTGCCCTTTCCCCAC26130.18026681972904096No Hit
GATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAAAA24470.1688147370367253No Hit
GTTTTAAGTACCGCTGATTTTCTTGCAGCTATTTGCCTAATTCCTCTGAC23730.16370959174015084No Hit
GCCTAGTAGTAGGAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCCA23610.16288173034070635No Hit
GAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGT23500.16212285739121557No Hit
ATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCATGCTTAACACC23490.16205386894126184No Hit
GAGCAAAAGCAGGGAACAAAGGGACAGAAACCAGAGCCTCAAGAGCTTAC21380.1474973060010293No Hit
CTTAAAGACAGTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGG21290.14687640995144594No Hit
GATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAG21280.14680742150149223No Hit
GCAAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTGC21260.14666944460158482No Hit
GGCTAAGACAGGAACAGGCAGGACACAATTGTTACTTGACGGCCCTGCCT20620.14225418380454743No Hit
ACCATAAACACTGGAGATGGGGAATTATGAAATGGTATGAGTGGGGAAAG20530.14163328775496406No Hit
GCATTAGGCTGAGGGATGGGCCTAGTAGTAGGAATAAAAGAGGGATAAGA20330.14025351875588987No Hit
GATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAG18540.12790458621417602No Hit
GTCTAAGCTCACACAGCCAGGTGGAGAGGAGCAGAGCTGAGATTCAAGCC18270.1260418980654259No Hit
TGATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCA18220.12569695581565735No Hit
TTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATCACCAGCACCAATT17900.12348932541713865No Hit
CCTATGAATCCCTCCAGGACAGTCATCCACCTCAGGTAGAAAGTTTCTGC17850.12314438316737013No Hit
GTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGGTAATTACAGA17780.12266146401769416No Hit
GAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAA17590.12135068346857368No Hit
ATTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTG17580.12128169501861999No Hit
GTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTGATTATACTGT17460.12045383361917547No Hit
ATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGG17320.11948799531982357No Hit
CCAGACCCTCAAGAGCTTACATTCTAATGAGAGGGAGACACGTACTGAAC17280.11921204152000872No Hit
GCTCCATACCCAGCACATAAACTTAATATTATTCCCCACACAGGCCACAA17270.11914305307005502No Hit
GAGCAAAAGCAGGGAACAAAGGGACAGAAACCAGACCCTCAAGAGCTTAC17210.11872912237033276No Hit
TCATATCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTGCC16900.11659048042176778No Hit
CTCATAGTCCTAGCTACTTTAGGAGGCTGAGGCAGAAGGATTGCTTAAGC16880.11645250352186037No Hit
ACTTAATATTATTCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAAT16870.11638351507190665No Hit
GGATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGA16730.11541767677255474No Hit
GGCCACAAGGTGGGAAGTAGGAGATAATGACTCCCACTTGTGTATTGATA16620.11465880382306394No Hit
CCACAGAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAG16280.11231319652463784No Hit
AGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTC16120.1112093813253785No Hit
GGTATGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGT15950.11003657767616547No Hit
TTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTGA15860.10941568162658208No Hit
CTTCTGTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTGATTAT15790.10893276247690611No Hit
GGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGA15420.1063801898286189No Hit
GTTTATCACCAGCACCAATTCACAAGGATGAGCCTGAGGCTCAGAATGGT15130.10437952477996135No Hit
CTTCATATCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTG14930.10299975578088717No Hit
GACCCTCAAGAGCTTACATTCTAATGAGAGGGAGACACGTACTGAACAAG14820.10224088283139637No Hit
CTTGTGTATTGATAGGATAAATACCTTACTTAGTTCAGTTTATACAGCCT14720.10155099833185927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)