FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_C02_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_C02_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210249
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACCATAAACCATAGAGCAATGGTTAAAACTCAAAACCAAATGAAGC19120.15798401816485697No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG18930.156414093298156No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA18260.15087804245242095No Hit
GTAGTAACAAGAGCCAACACCACAGCCATCTCAATTGGTTAAATTTACAC15300.12642026558171088No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG13680.11303459040247088No Hit
CAGACACCTGGGTCTCTGCTACCAACAATACGAGCTAATGTATAATGATT12620.10427606219877066No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)