FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_C02_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_C02_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2974237
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA75490.25381299472772345No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATTCCGAATATC62990.2117854091654431No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG61350.20627138993967192No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT52210.17554081937653254No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG49900.16777412156462312No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA47350.15920049410991793No Hit
TCTCTGTACACATCGTGATCATAAGTTCCATTTCTGATTGATCCTATGCA46350.1558382872649355No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA45550.15314852178894958No Hit
CTTTTAAGGCCATGTTTGAAGAGTCGATAGATACATTTGAAAAAAAGACG45120.15170277284560713No Hit
GTACGTGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT43110.14494473708719244No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG42570.14312914539090194No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT41360.1390608751084732No Hit
CCCTAGAAGATCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA39440.13260543796610694No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG38760.1303191373115189No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT38540.12957945180562275No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACCATCATTGCTTT37950.12759574976708313No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT36890.12403181051140176No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT36630.12315763673170631No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG36590.12302314845790703No Hit
GTCCCAGATATGTCAAGCAAAACACTCTGAAATTGGCAACAGGAATGAGA36450.12255243949960948No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG34580.11626511269949234No Hit
GTTCGTGTACTTCGTTGAAACTTTAGCTAGAAGCATTTGTGAAAAGCTTG33560.11283566171761027No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG33240.11175975552721588No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT32470.10917085625657941No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC31720.1066492011228426No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA31590.10621211423299488No Hit
ATCACGTACTGTGTTATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC31300.10523707424794997No Hit
GAATAGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAAAAAG30350.10204297774521667No Hit
GTGTTAATGTATACCCCCTTCATTATGTATTCAGTAGCTCTACAATGGGA30300.10187486740296754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)