FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_C02_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_C02_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986254
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATTCCGAATATC26280.2664627976160299No Hit
TCTCTGTACACATCGTGATCATAAGTTCCATTTCTGATTGATCCTATGCA22980.2330028572761175No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG18940.19203977879937623No Hit
GAATAGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAAAAAG16480.1670969141823506No Hit
GTTCGTGTACTTCGTTGAAACTTTAGCTAGAAGCATTTGTGAAAAGCTTG15710.15928959476970436No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT14780.1498599752193654No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA14360.145601437357922No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG13960.1415456870136902No Hit
GTCCCAGATATGTCAAGCAAAACACTCTGAAATTGGCAACAGGAATGAGA13230.1341439426354671No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT12910.1308993423600817No Hit
CTTTTAAGGCCATGTTTGAAGAGTCGATAGATACATTTGAAAAAAAGACG12890.1306965548428701No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA12470.12643801698142668No Hit
ACCTTATATAGTCAAACCACACGAAAAGGGAATAAATTCAAATTACCTGC12280.12451153556791658No Hit
ATTCAGGACCTTGAGAAATATGTTGAGGACACTAAAATAGATCTCTGGTC12040.1220780853613775No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA11660.11822512253435728No Hit
CCCTAGAAGATCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA11620.1178195474999341No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG11460.11619724736224137No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG11230.11386519091430808No Hit
GAGTAAGGAATATCCCTAGCAGAGTAAGCATCTATTGGACAATAGTAAAA10950.11102616567334582No Hit
ATCCTATGCAGGCATTGTCACATTTGTGGTATATTTTGAAACAACCATTG10900.11051919688031683No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG10370.10514532767420968No Hit
CCATTATATGATGCGATCAAGTGCATAAAAACATTCTTTGGATGGAAAGA9990.10129236484718947No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT9910.1004812147783431No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT9880.10017703350252573No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)