FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_B08_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_B08_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827546
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC85420.4674027356903739No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTGTAGTAAGGACAATT39380.21548021226278297No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT31070.17000940058417136No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTATTGTCAGGACC30830.1686961641457999No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA30320.16590553671426053No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC29650.1622394183238069No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC29590.16191110921421403No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA29530.16158280010462117No Hit
AGCATATACTGACACATATCATTCCTATGCAAACAACATCCTAAGAACAC27970.15304676325520672No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT26500.14500319007018156No Hit
TATCATTCCTATGCAAACAACATCCTAAGAACACAAGAAAGTGCCTGCAA24800.13570109863171706No Hit
CCCCCAGACCAGATGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG23980.13121420746728127No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA23960.13110477109741697No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC22940.12552351623433827No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG22650.1239366888713061No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT22430.12273288880279895No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT21560.11797240671370243No Hit
ATACTGAGGAGTGCACATGCGGATTTGCCAGCAATAAAACCATAGAATGT21480.11753466123424526No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA20130.11014770626840582No Hit
GTAATATTCAGTGAATCAAAAGTGGGGAGAGAAAATTCTCCTGCATCAAA20000.10943636986428795No Hit
GTTTAATATGCTATCCACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA19400.10615327876835932No Hit
AACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACA19100.104511733220395No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT19030.10412870592586998No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT18990.10390983318614143No Hit
GGAATGATATGTGTCAGTATATGCTTCTCCATATTTTATTTTGAGCAATG18840.10308906041215926No Hit
GCATTATTGTCAGGACCATCAACTCCGATATATGTCCATTCCTTACCATC18660.10210413308338066No Hit
CAATAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACAT18340.10035315116555205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)