FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_B06_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_B06_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1667008
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA52210.3131958574883864No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG49920.299458670864207No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT40000.23995085806426844No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG36730.2203348754175145No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG28070.16838551464660037No Hit
CAATAGGATGGGGGCTGTAACCACCGAAGTGGCATTTGGCCTGGTATGTG27030.16214679233692939No Hit
CTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC26940.1616069029062848No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA26780.16064709947402772No Hit
TCTCTGTACACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCA26770.16058711175951165No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG24280.14565017084501095No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT22870.13719190309824547No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA22850.13707192766921333No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT22830.13695195224018122No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA22500.134972357661151No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG22240.13341267708373325No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC21870.13119313164663876No Hit
CTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA21680.13005336507083348No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT21610.12963345106922103No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA21270.12759386877567475No Hit
CCTCTTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG21230.12735391791761047No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA20750.12447450762083925No Hit
ATATACAATAGGATGGGGGCTGTAACCACCGAAGTGGCATTTGGCCTGGT20300.12177506046761623No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA20260.12153510960955197No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGACTCCAACACTGTGTCAAG19920.11949552731600568No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAACAA19850.11907561331439322No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGAATGAGA19740.11841574845471647No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG18380.11025741928053134No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG18170.10899767727569395No Hit
GTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACCAGGCCAAATG18000.10797788612892079No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA17670.10599829154989057No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC17410.10443861097247284No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGAATTACAGCCTGTTGGCTT17380.10425864782892463No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC17220.10329884439666756No Hit
GAATAGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAAAAAG17070.10239902867892656No Hit
GAGTAAGGAATATCCCTAGCAGAGTAAGCATCTATTGGACAATAGTAAAA16870.10119927438860521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)