FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_B03_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_B03_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2479210
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC107210.43243613893135313No Hit
GTTGTAAAGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT98060.3955292210018514No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG97500.39327043695370706No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG68980.2782337922160688No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT61080.24636880296546076No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA60540.2441906897761787No Hit
GTTCTACTTCAAGCAGTAGTTGTAAAGCTTGCATAAATGTTATTTGTTCG57150.23051697919901906No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT56270.2269674614090779No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA54310.2190617172405726No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT54130.2183356795108119No Hit
ACTTCAAGCAGTAGTTGTAAAGCTTGCATAAATGTTATTTGTTCGAAACT52900.21337442169078055No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAAGCTTGCATAAATGTTATTT50670.2043796209276342No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC49790.20083010313769306No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGGGGACTTGAA49140.19820830022466832No Hit
GTGTTACCATTCCATTCAAGTCCCCCGATGAGGACCCCAACTGCATTTTT48770.19671588933571582No Hit
ATCTACTACTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG46250.18655136111906614No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT45810.1847766022240956No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT45770.18461526050637098No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAAGCTTGCATAAA41460.16723069042154556No Hit
CATTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA39340.1586795793821419No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC38420.15496871987447614No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA37780.15238725239088258No Hit
ATCTTCAACCGATTAGAGACATTGATACTACTAAGGGCTTTCACTGAGGA36010.14524788138156913No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCATCTTATTTCCTCAAAT33990.13710012463647694No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGATTAGAGACATTGAT33780.1362530806184228No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC33630.13564804917695555No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT33480.13504301773548832No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA33390.13467999887060797No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC33240.1340749674291407No Hit
GTAGTTGTAAAGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC33150.13371194856426039No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGATTAGAGACA32970.13298591083449968No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC32960.13294557540506854No Hit
TGGTAACACAGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA31610.12750029243186337No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT30500.12302305976500578No Hit
CTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAAGCTTGCATAAATGT29950.12080461114629257No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC29710.11983656083994498No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAAGCTTGCATAAAT29210.11781978936838751No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG29010.11701308077976454No Hit
TCTCATTTCTGCTCTGGAGGTAATGAAGGTCTCCCATTCTCATCACAGCT28670.11564167617910545No Hit
ATTAGAGACATTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG28070.11322155041323648No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC28020.11301987326608072No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTCGGGTGTC27920.11261651897176922No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC27770.11201148753030199No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAA27330.11023672863533143No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT27260.10995438062931337No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGATT27040.10906700118182809No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC27000.1089056594641035No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC26640.1074535840045821No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG26470.10676788170425257No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC26250.10588050225676726No Hit
CCCTTAGTAGTATCAATGTCTCTAATCGGTTGAAGATTACACTGAAGTTC25170.10152427587820313No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA25090.10120159244275395No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)