FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_B03_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_B03_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255769
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG52960.4217336150199599No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC52200.415681546526471No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA37790.30093114259071535No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT31040.24717921847091304No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT28850.22973970531204388No Hit
GTTGTAAAGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT27720.22074123505198806No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT23820.18968456778276896No Hit
GTGTTACCATTCCATTCAAGTCCCCCGATGAGGACCCCAACTGCATTTTT22380.17821749063721112No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC20410.1625298920422466No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC18560.14779788320941192No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA18550.14771825072923445No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA17980.1431791993591178No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC17770.1415069172753906No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG17320.1379234556674038No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT16240.12932314780823542No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT15510.12350997675527903No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGGGGACTTGAA15060.11992651514729222No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC14970.11920982282569484No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC14920.11881166042480742No Hit
ACTTCAAGCAGTAGTTGTAAAGCTTGCATAAATGTTATTTGTTCGAAACT14900.11865239546445246No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC14890.11857276298427497No Hit
GTTCTACTTCAAGCAGTAGTTGTAAAGCTTGCATAAATGTTATTTGTTCG14650.11666158346001533No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT14590.11618378857895043No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT14420.11483003641593319No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG14320.11403371161415833No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT13820.1100520876052841No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC13800.10989282264492913No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAAGCTTGCATAAATGTTATTT13560.10798164312066949No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT13200.10511487383428003No Hit
CCCTTAGTAGTATCAATGTCTCTAATCGGTTGAAGATTACACTGAAGTTC12980.10336295927037537No Hit
GTATCAATGTCTCTAATCGGTTGAAGATTACACTGAAGTTCGCTTTCAGT12980.10336295927037537No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG12910.10280553190913297No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC12820.10208883958753562No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC12710.10121288230558326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)