FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_B01_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_B01_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431256
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCTCTGGATTTTTAGTCAAATTTCTTCAAAGCCAGTTTGAAATGAGC31050.21694232198851918No Hit
ATTTTGTAGTGATGGAGGGCAGTTGAATAATTTTAAAAAGGGGAATTACA29750.20785939063312225No Hit
CACGTGCAGTTTGATATTACTGAACCTAAAGGCAAAGCTGCAATGTGGAG29220.20415634938822963No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG27010.18871536608405484No Hit
GTTTGATATTACTGAACCTAAAGGCAAAGCTGCAATGTGGAGAGATGAAG25810.18033112175599614No Hit
CACGAGCAGTTTGATATTACTGAACCTAAAGGCAAAGCTGCAATGTGGAG20890.14595572001095541No Hit
TTATTAAATCATGCCACATTATTTTGTAGTGATGGAGGGCAGTTGAATAA19910.13910858714304078No Hit
ATATTACTGAACCTAAAGGCAAAGCTGCAATGTGGAGAGATGAAGGAAAA19480.13610423292548643No Hit
GTGGAGAGATGAAGGAAAAGAGAGAGCCAAATAGATTATTAAATCATGCC17180.12003443129670723No Hit
GTAGTAACAAGAGCCAGCACGCTGTGACATATCGATGTTGTGATGAGCTT16680.11654099616001609No Hit
GTAGTAACAAGAGCAGTTTGATATTACTGAACCTAAAGGCAAAGCTGCAA15950.11144058086044704No Hit
GTAGAAACACGAGCAGTTTGATATTACTGAACCTAAAGGCAAAGCTGCAA15110.10557160983080596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)