FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_A12_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_A12_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071600
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA18840.17581187010078386No Hit
GTGTAATGATAAAAGGAATGAGGGAGGCTGATAAATACAGATCTGTATGT18610.17366554684583801No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC17630.16452034341172078No Hit
GAGCAAAAGCAGGTGCAGGATAAATGAAGGAACTGAACCAGCTAGGACCA15770.14716312056737588No Hit
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC15010.1400709219858156No Hit
CTCATATACAGAGCTACAATCAACCAACACTCCCATCACCACCAACTGCC14360.1340052258305338No Hit
GATAAATACAGATCTGTATGTCTTCCAGTTTTAGCCAGCTTGTCTTGTTT14080.13139231056364314No Hit
CCACACTGGCAAACCCTAAGGCCCCTTGCCAGTGCTCACCTTGCGTGGCC13550.12644643523702873No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC13360.12467338559163867No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG13010.12140724150802538No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA11910.11114221724524076No Hit
GTATTTACCACTCACACATCTGAGGAGAGAAAGTAGGAGGTGCTGGGGGG11440.10675625233296006No Hit
GGATAAATGAAGGAACTGAACCAGCTAGGACCAAAATCAGTAATTTGAAC11140.10395670026129153No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA10990.10255692422545726No Hit
ACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAGTCATGTTAAG10810.10087719298245615No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)