FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_A11_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_A11_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116864
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT61420.5499326686149791No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT34870.3122134834679961No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG34430.3082738811529425No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA33700.30173772276660366No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT31300.2802489828663114No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT29520.26431150077359467No Hit
ATCTACTACTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG29260.261983553951063No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC28580.2558950776459802No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC26570.2378982579794854No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG24860.22258753080052718No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC24440.21882700131797606No Hit
CATTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA22360.2002034267377228No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT22000.1969801157526789No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT21960.19662197008767407No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA20260.18140077932496704No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC20170.1805949515787061No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCATCTTATTTCCTCAAAT19090.1709250186235746No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT18980.16994011804481118No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGATTAGAGACA18740.16779124405478196No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT18690.16734356197352587No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG18600.1665377342272649No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA18420.164926078734743No Hit
ATCTTCAACCGATTAGAGACATTGATACTACTAAGGGCTTTCACTGAGGA18100.16206091341470402No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA18050.16161323133344793No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGATTAGAGACATTGAT17670.15821084751590167No Hit
TGGTAACACAGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA16610.14871998739327258No Hit
GTGTAATCTTCAACCGATTAGAGACATTGATACTACTAAGGGCTTTCACT14830.13278250530055585No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGATT14830.13278250530055585No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC14720.13179760472179244No Hit
CGATTGGACCAGGCGGTCATGGAAAAGAACATAGTACTGAAAGCGAACTT14720.13179760472179244No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTCGGGTGTC14340.12839522090424618No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT14070.1259777376654633No Hit
TCTCATTTCTGCTCTGGAGGTAATGAAGGTCTCCCATTCTCATCACAGCT13800.12356025442668044No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGATTAGAGA13540.12123230760414876No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG13390.1198892613603805No Hit
CTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT13330.1193520428628732No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA13090.11720316887284396No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA12850.11505429488281474No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAA12300.11012979198899775No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA12150.1087867457452295No Hit
ATGTAGGACCATGAACTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC12150.1087867457452295No Hit
ATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTC12070.10807045441521976No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT12030.1077123087502149No Hit
GTGCCGCATTTCTTCAATTAACCATCTTATTTCCTCAAATTTCTGTCCCA11950.10699601742020515No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT11920.10672740817145149No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA11780.10547389834393445No Hit
CTTCAGTGTAATCTTCAACCGATTAGAGACATTGATACTACTAAGGGCTT11620.10404131568391495No Hit
GTATATTTTCAGAGACTCGAACTGTGTTACCATTCCATTCAAGTCCTCCG11590.1037727064351613No Hit
ATTAGAGACATTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG11540.10332502435390523No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCATCTTA11520.1031459515214028No Hit
GTCATGGAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTCAA11420.1022505873588906No Hit
CATTATGTATAGGGTATCATGCGAACAATTCAACAGACACTGTAGACACA11370.10180290527763453No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)