FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_A06_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_A06_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433459
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCATATACTGCCTAGTAGACATCCGCACATGGAGGACATGAAGGCATC28960.2020287988704246No Hit
CACCTGAGCCTCCTTGGTGGCTGATCTCACCTGAGCCTCCTTGGTGGCTG23700.16533434161702568No Hit
ATATAAAGCACTTACTTCCACAGTGCCTGGCAAGTGTTGGAGATCATTAA23470.16372983112875916No Hit
GGTGAGATCAGCCACCAAGGAGGCTCAGGTGAGATCAGCCACCAAGGAGG22010.1535446775945458No Hit
GATCTCACCTGAGCCTCCTTGGTGGCTGATCTCACCTGAGCCTCCTTGGT20760.14482451189744527No Hit
AAACTAAAGTGCCTTTACCATCTTTCCTCCAATTTTAGGCCTTCTTTGTC18240.12724465785209066No Hit
AGCACGCACTTACCAGGTGGTGATGGAGCTAATGGGAGCCAGGTCAAATG18010.12564014736382415No Hit
TTATAGATGAGTTCATATACTGCCTAGTAGACATCCGCACATGGAGGACA17910.12494253410805611No Hit
CCTTGGTGGCTGATCTCACCTGAGCCTCCTTGGTGGCTGATCTCACCTGA16740.11678045901557003No Hit
AGCACGCACTTAGCCGCAGTCTATCAACTAGCTGAGGCAGAACGTGCCGC14970.1044327043884757No Hit
CCAATATACATCCTCGGGACAGCCATTATAACCCCCTGATGATGTTGGTA14390.10038654750502109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)