FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_A06_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_A06_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412649
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATTCCCTGAGGACATGGAAATTAGGGGAAGCAATTTACATTCCCT63290.448023535924352No Hit
ACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAGTCATGTTAAG40110.2839346504333348No Hit
ATATATTATTCCCTGAGGACATGGAAATTAGGGGAAGCAATTTACATTCC35710.25278749356705027No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA26610.1883695100481436No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA24800.1755567023372402No Hit
GCATAAGATGCAATAAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCA22830.16161127074029005No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC20670.14632084827865946No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA19400.13733064618316368No Hit
GTTAAGGGCAGAGGCTCACTGGGGTAAGTGGCAGGGCTGAAGCAGGTGGA19390.13725985719028577No Hit
CTAAAGAAGAGCCCTTGTCAAGGTTACCAGCAACCTCCACATTGGCAAAC18680.13223383869595348No Hit
GTATGGGGAGGATCTGAAAATTAAACAAACAAATTTGCAGCAATATGCAC18340.12982701293810422No Hit
ACTTGACACTGTCTGAAGACATTTTTGGTTGTCACTATCTGGGGAGAAGG17590.12451783847226028No Hit
GTCCAGGGTCTAGAGAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGG16950.11998734292807342No Hit
GCCTAGAACCAGGAGTTCTAGTCTGATGGACAGCCTTTCATCTGGAAGTC16760.11864235206339296No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC16720.11835919609188128No Hit
GTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGCAGGGCTGAAGCAG16390.11602315932690994No Hit
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA15790.11177581975423477No Hit
TCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGAGG15570.11021846191092055No Hit
ATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGT15300.10830715910321671No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT14850.10512165442371035No Hit
CTTCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGA14580.10321035161600652No Hit
TCTCAAGGTAATATATGTGGACTTCTCTCCTTGTCACCCCAATTTCGATG14500.10264403967298318No Hit
CTATATGATAAACGGTCAACCTTACTAATCTTAAGATTTCTCAGGCTTGA14160.10023721391513392No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)