FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_A06_FluA_Rep1_Plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_A06_FluA_Rep1_Plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340818
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGAGGGATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCA103410.7712456127528121No Hit
GTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATC98210.7324633171690714No Hit
GATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACGA88240.6581057235210148No Hit
TCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAATGACTCCCACTTG72280.5390739086139953No Hit
ATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTGCTGAGCAAGGCAGG63070.4703844966281777No Hit
GTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTGAAGAAAGACAGTTT61270.4569598558491906No Hit
GCCTAGTAGTAGGAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCCA51250.3822293555128288No Hit
CTCCAGGACAGTCATCCACCTCAGGTAGAAAGTTTCTGCCCTTTCCCCAC50050.37327959499350394No Hit
GTTTTAAGTACCGCTGATTTTCTTGCAGCTATTTGCCTAATTCCTCTGAC46660.3479965215264115No Hit
GAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGT45660.3405383877603075No Hit
ATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCATGCTTAACACC44260.3300970004877619No Hit
GATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAAAA43760.32636793360470995No Hit
TGGTATGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTG41180.30712594848816166No Hit
TTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATCACCAGCACCAATT39810.2969083052285993No Hit
GTCTAAGCTCACACAGCCAGGTGGAGAGGAGCAGAGCTGAGATTCAAGCC39770.2966099798779551No Hit
GCATTAGGCTGAGGGATGGGCCTAGTAGTAGGAATAAAAGAGGGATAAGA38810.2894501714624953No Hit
GATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAG38190.2848261285275108No Hit
GGTATGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGT37990.28333450177429004No Hit
TGATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCA37500.2796800162288991No Hit
CTTAAAGACAGTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGG37320.27833755215100037No Hit
GATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAG36790.27438474125496526No Hit
GGCTAAGACAGGAACAGGCAGGACACAATTGTTACTTGACGGCCCTGCCT35210.26260088990452096No Hit
ATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGG34720.25894640435913No Hit
GGATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGA33700.251339107917704No Hit
GGCCACAAGGTGGGAAGTAGGAGATAATGACTCCCACTTGTGTATTGATA33510.24992206250214422No Hit
GTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTGATTATACTGT33420.24925083046319485No Hit
CCTATGAATCCCTCCAGGACAGTCATCCACCTCAGGTAGAAAGTTTCTGC32710.24395555548926107No Hit
CCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGGTATGAGT32570.2429114167620065No Hit
CCACAGAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAG32390.24156895268410777No Hit
GAGCAAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCT32220.24030106994387007No Hit
CTTCTGTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTGATTAT32010.23873486185298823No Hit
CTTGTGTATTGATAGGATAAATACCTTACTTAGTTCAGTTTATACAGCCT31380.23403623758034273No Hit
AGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTC31310.23351416821671545No Hit
CTCACACCATTTCATAATTCCCCATCTCCAGTGTTTATGGTCTTACAGGT31280.23329042420373236No Hit
GCTCCATACCCAGCACATAAACTTAATATTATTCCCCACACAGGCCACAA30630.22844263725576475No Hit
GTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGGTAATTACAGA29740.2218048982039322No Hit
ACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACT29600.22076075947667764No Hit
GTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTCC29560.22046243412603353No Hit
GAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAA28970.21606213520403214No Hit
TCATATCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTGCC28800.2147942524637945No Hit
GTGTATTGATAGGATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGT28640.21360095106121788No Hit
GTTTATCACCAGCACCAATTCACAAGGATGAGCCTGAGGCTCAGAATGGT28520.2127059750092854No Hit
CTTCATATCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTG28220.21046853487945416No Hit
GTGGATGACTGTCCTGGAGGGATTCATAGGAGAGGCACATTTCAGGCCAA27550.20547158525616455No Hit
GTGCAGCCAAGGCAGGTAGAGCAAAAGAGCATGAGTGCTGAAGTCTGACA27110.20219000639907875No Hit
GGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGA26180.1952539419966021No Hit
ACTTAATATTATTCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAAT25940.19346398989273714No Hit
GAATGGTGAAGCACCTTGTCTAAGCTCACACAGCCAGGTGGAGAGGAGCA25130.1874229015421929No Hit
TTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGCTAGTT25080.1870499948538877No Hit
GTGGTAGTGTGTTCTCCACGTCATTTCTGTGACAATCAAATGAGATCACA24970.18622960013961629No Hit
CTTCTCTTCTGTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTG24620.18361925332147985No Hit
GCATTCACCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGG24450.18235137058124218No Hit
GGATGAGCCTGAGGCTCAGAATGGTGAAGCACCTTGTCTAAGCTCACACA24420.1821276265682591No Hit
GTGCTGAAGTCTGACAAACCAGGGCTTGAATCTCAGCTCTGCTCCTCTCC24180.18033767446439414No Hit
TTTCAGGCCAAGAGAGTTTCATGCTTAACACCACAGAGATATGAAGATCA24090.17966644242544477No Hit
GTGAAGCACCTTGTCTAAGCTCACACAGCCAGGTGGAGAGGAGCAGAGCT24030.17921895439947852No Hit
CCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGGTGTGAGT23740.17705609560730837No Hit
CACTTAAGGGGAGGCAGGCATTAGGCTGAGGGATGGGCCTAGTAGTAGGA23640.17631028223069797No Hit
ACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAG23630.17623570089303695No Hit
CTTAAGGGGAGGCAGGCATTAGGCTGAGGGATGGGCCTAGTAGTAGGAAT23280.17362535407490054No Hit
GTACTGAACAAGTAATTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAG23190.1729541220359512No Hit
ATATTATTCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAATGACTC23190.1729541220359512No Hit
TCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTGCCTCTCC22710.1693742178282213No Hit
GTGTTTATCACCAGCACCAATTCACAAGGATGAGCCTGAGGCTCAGAATG22150.16519766291920307No Hit
GCTTAACACCACAGAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAG22000.16407894285428745No Hit
CTTCAGCACTCATGCTCTTTTGCTCTACCTGCCTTGGCTGCACAGATGCC21730.16206524673743938No Hit
AGCTTAGACAAGGTGCTTCACCATTCTGAGCCTCAGGCTCATCCTTGTGA21670.16161775871147316No Hit
TCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAAAAGAGCATGAGT21550.16072278265954065No Hit
CAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCT21080.1572174597894718No Hit
TCACCATTCTGAGCCTCAGGCTCATCCTTGTGAATTGGTGCTGGTGATAA21050.15699371577648868No Hit
ACCATAAACACTGGAGATGGGGAATTATGAAATGGTATGAGTGGGGAAAG20810.15520376367262373No Hit
GGCCAAGAGAGTTTCATGCTTAACACCACAGAGATATGAAGATCACTGAG20760.15483085698431853No Hit
GCCCAATAAGCAGATGTTCTGGTGGTGAAGACGACAAAGCATTCACCTGT20710.15445795029601334No Hit
GTATAAACTGAACTAAGTAAGGTATTTATCCTATCAATACACAAGTGGGA20700.1543833689583523No Hit
AACTAGCAACGTACTTAAAGACAGTATAATCACACCAAGCTCCTAGTAAA20580.1534883929064198No Hit
CAATTGTTACTTGACGGCCCTGCCTCTTTGGGCATAAAGCCCCGCACCTC20330.15162385946489382No Hit
GTGCTTCACCATTCTGAGCCTCAGGCTCATCCTTGTGAATTGGTGCTGGT19470.1452098644260444No Hit
GCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTCCTTCCCCACC19270.1437182376728236No Hit
ATTCACAAGGATGAGCCTGAGGCTCAGAATGGTGAAGCACCTTGTCTAAG19190.1431215869715353No Hit
TTGTGGTAGTGTGTTCTCCACGTCATTTCTGTGACAATCAAATGAGATCA19090.1423757735949249No Hit
GTCTTACAGGTGAATGCTTTGTCGTCTTCACCACCAGAACATCTGCTTAT18950.14133163486767034No Hit
ATTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGCTAGT18890.14088414684170408No Hit
GTACTGAACAAGTAATTTCGGTGTGCTAGTTTAGAAGTGGGTTCGTATAG18850.14058582149105994No Hit
CCTCTCCACCTGGCTGTGTGAGCTTAGACAAGGTGCTTCACCATTCTGAG18760.13991458945211058No Hit
CCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACGACAAAGCATTCA18620.13887045072485601No Hit
GTATTTATCCTATCAATACACAAGTGGGAGTCATTATCTCCTACTTCCCA18610.138795869387195No Hit
GTTTATGGTCTTACAGGTGAATGCTTTGTCGTCTTCACCACCAGAACATC18490.13790089333526248No Hit
GGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACGACAAAGC18260.13618552256905858No Hit
CTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATCAC17980.13409724511454948No Hit
GCCTAATTCCTCTGACATTTGTTCATTCACATAATGTTCCAGAAACACTT17820.13290394371197284No Hit
GCCAGGTGGAGAGGAGCAGAGCTGAGATTCAAGCCCTGGTTTGTCAGACT17650.13163606097173516No Hit
GGGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTG17640.13156147963407414No Hit
TCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCATGCTTAACACCAC17610.131337735621091No Hit
GAATACTACCAGGTGTTTATCACCAGCACCAATTCACAAGGATGAGCCTG17580.13111399160810788No Hit
ACACCACAGAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGG17300.1290257141535988No Hit
GACCCTCAAGAGCTTACATTCTAATGAGAGGGAGACACGTACTGAACAAG17280.12887655147827667No Hit
GTGGAGAGGAGCAGAGCTGAGATTCAAGCCCTGGTTTGTCAGACTTCAGC16960.1264899486731234No Hit
CCAGACCCTCAAGAGCTTACATTCTAATGAGAGGGAGACACGTACTGAAC16890.12596787930949616No Hit
GAGCAAAAGAGCATGAGTGCTGAAGTCTGACAAACCAGGGCTTGAATCTC16820.12544580994586887No Hit
GAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGTCCTGG16690.12447625255627534No Hit
GAAGAAAGACAGTTTTAAGTACCGCTGATTTTCTTGCAGCTATTTGCCTA16600.123805020517326No Hit
GAGCAGAGCTGAGATTCAAGCCCTGGTTTGTCAGACTTCAGCACTCATGC16600.123805020517326No Hit
CCATACCCAGCACATAAACTTAATATTATTCCCCACACAGGCCACAAGGT16580.1236558578420039No Hit
GAGCAAAAGCAGGGAACAAAGGGACAGAAACCAGACCCTCAAGAGCTTAC16470.12283546312773247No Hit
GGTGTGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGT16440.12261171911474936No Hit
GACACGTACTGAACAAGTAATTTCAGTGTGCTAGTTTAGAAGTGGGTTCG16200.1208217670108844No Hit
GGATATTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGC16160.12052344166024025No Hit
ATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAA16100.120075953634274No Hit
CCACCTGGCTGTGTGAGCTTAGACAAGGTGCTTCACCATTCTGAGCCTCA16090.12000137229661297No Hit
GTTTCATGCTTAACACCACAGAGATATGAAGATCACTGAGGCATCTGTGC16050.11970304694596881No Hit
ACCATAAACACTGGAGATGGGGAATTATGAAATGGTGTGAGTGGGGAAAG15990.11925555892000257No Hit
GAACAAAGGGACAGAAACCAGACCCTCAAGAGCTTACATTCTAATGAGAG15980.11918097758234153No Hit
GAGCAAGGCAGGGGCTAAGACAGGAACAGGCAGGACACAATTGTTACTTG15880.11843516420573114No Hit
GAAAAAAACATCCTGAAGAAAGACAGTTTTAAGTACCGCTGATTTTCTTG15870.11836058286807008No Hit
GCACATAAACTTAATATTATTCCCCACACAGGCCACAAGGTGGGAAGTAG15860.11828600153040905No Hit
GTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTGCCTCTCCTATGAATC15830.11806225751742593No Hit
GCCTCTTTGGGCATAAAGCCCCGCACCTCACTCAGCTTCATTTGTAAAGT15810.11791309484210385No Hit
GGGATGGGCCTAGTAGTAGGAATAAAAGAGGGATAAGATATGGGGTTCCT15750.11746560681613762No Hit
GACAGGAACAGGCAGGACACAATTGTTACTTGACGGCCCTGCCTCTTTGG15720.11724186280315449No Hit
CTTTTGCTCTACCTGCCTTGGCTGCACAGATGCCTCAGTGATCTTCATAT15680.11694353745251033No Hit
AGTAATTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAGAAAAAAACAT15640.11664521210186618No Hit
CAGTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGGTAATTACA15460.11530274802396746No Hit
GTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTCCTTCCCCACCACTTC15440.11515358534864538No Hit
TGGCTGCACAGATGCCTCAGTGATCTTCATATCTCTGTGGTGTTAAGCAT15240.11366195859542458No Hit
ATTCTAATGAGAGGGAGACACGTACTGAACAAGTAATTTCAGTGTGCTAG15240.11366195859542458No Hit
GCTCTGCTCCTCTCCACCTGGCTGTGTGAGCTTAGACAAGGTGCTTCACC15150.11299072655647524No Hit
CCACTTCTCTTCTGTAATTACCATCATGGGATATTTACTAGGAGCTTGGT15040.11217033184220378No Hit
GGCTCATCCTTGTGAATTGGTGCTGGTGATAAACACCTGGTAGTATTCTC15030.11209575050454275No Hit
ATTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTG15010.11194658782922068No Hit
TCTCAGCTCTGCTCCTCTCCACCTGGCTGTGTGAGCTTAGACAAGGTGCT14820.11052954241366092No Hit
GCACAGATGCCTCAGTGATCTTCATATCTCTGTGGTGTTAAGCATGAAAC14820.11052954241366092No Hit
CTCAAGAGCTTACATTCTAATGAGAGGGAGACACGTACTGAACAAGTAAT14810.11045496107599988No Hit
CCTAGTAAATATCCCATGATGGTAATTACAGAAGAGAAGTGGTGGGGAAG14730.10985831037471155No Hit
CTTTCTACCTGAGGTGGATGACTGTCCTGGAGGGATTCATAGGAGAGGCA14350.10702421954359204No Hit
GGCCTAGTAGTAGGAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCC14250.10627840616698164No Hit
AGCGAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTG14230.10612924349165957No Hit
CTCATAGTCCTAGCTACTTTAGGAGGCTGAGGCAGAAGGATTGCTTAAGC14030.10463761673843877No Hit
GTGTGAGCTTAGACAAGGTGCTTCACCATTCTGAGCCTCAGGCTCATCCT14000.10441387272545564No Hit
ATATTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGCTA13960.10411554737481149No Hit
AGATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACG13930.10389180336182838No Hit
GCCTGAGGCTCAGAATGGTGAAGCACCTTGTCTAAGCTCACACAGCCAGG13760.10262392062159069No Hit
GTAGGAGATAATGACTCCCACTTGTGTATTGATAGGATAAATACCTTACT13740.10247475794626863No Hit
GGTGATAAACACCTGGTAGTATTCTCGAACTAACTAGCAACGTACTTAAA13680.10202726992030238No Hit
GAACAAAGGGACAGAAACCAGAGCCTCAAGAGCTTACATTCTAATGAGAG13680.10202726992030238No Hit
GATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCATGCTTAACAC13660.1018781072449803No Hit
AGCTCACACAGCCAGGTGGAGAGGAGCAGAGCTGAGATTCAAGCCCTGGT13500.10068480584240368No Hit
CTCTTGGCCTGAAATGTGCCTCTCCTATGAATCCCTCCAGGACAGTCATC13420.10008815514111534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)