FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_A03_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_A03_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200106
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT34520.2876412583555119No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA24110.2008989205953474No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAATAACT23210.19339958303683175No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA17780.14815357976712057No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG17500.14582045252669348No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT16540.13782115913094345No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT15160.12632217487455275No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT15050.12540558917295638No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT15020.1251556112543392No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC13470.11224008545911777No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT12740.10615728943943285No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGTGTTCCTAGTT12360.1029909024702818No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC12130.10107440509421668No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGT12010.10007449341974792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)