FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_H10_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_H10_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778352
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC26330.33827882500462514No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA13710.17614138590252226No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT12050.15481427426151664No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC10940.14055337430879603No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG10640.13669907702427694No Hit
CTGTAGGGAAATCCAGGAGATAGTTAAACACAGGCAAGGGGCTGGAGCTC9840.12642095093222605No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG9570.12295208337615886No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA9330.11986864554854358No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA9070.11652825456862703No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA8960.11511501223097004No Hit
GAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGTTTGA8870.11395872304561432No Hit
GCCAACAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGG8740.11228852755565605No Hit
TTCTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC8510.10933356630419142No Hit
GTTCTGGAGCACGGGGCCCAGCACAGGGTGAGAGGCAAGATGCCTGTGGG8020.10303821407281023No Hit
GCAGGCAGGATGTGAGGTTGCTCCCTCTCCACCTGCTTCAGCCCTGCCAC7930.10188192488745451No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC7790.10008325282134561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)