FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_H08_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_H08_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102291
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC58110.5271747660100644No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA38690.3509962432787712No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA28410.25773593361462627No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG22540.20448320815465246No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATCATTGTCAGGGCC20630.18715566034740372No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA18250.16556426569753357No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT18150.1646570642416567No Hit
GGAACAGAGAGCCATTGCTAAGATCAAGATGTTGGGAAACATCAAATTCA17380.1576716130314046No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT16650.15104904240350325No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT16600.15059544167556482No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT16580.15041400138438943No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC16540.15005112080203867No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC15910.14433575163001422No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC15710.14252134871826042No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT15570.14125126668003277No Hit
TCATTGGATTGTGCAAACGCATCAAATGTTCAGGCTGTGAACCGTTCTGC14760.1339029348874299No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT14170.12855044629775622No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC13800.1251938009110117No Hit
GTCTATGATAAGCGTGAAAATCCCCTCCTCCCCGAGGAGGAGGAACAGAG13490.12238147639779332No Hit
TCCTTCGACTGTCCTTGCAGCTGGGATAAGAGTCCCTGACTCTGGTTATG13460.12210931596103025No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG13290.12056707348603952No Hit
CCATTATACTTGACATACAGTCCCATCCCCATCCTTTCAGTCTTAGACAT13240.12011347275810108No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG13080.11866195042869805No Hit
CATATCAGCAAGTCCTCCAAGTGGGTCCCTATCCATTTTCATCCTGGGTG12820.11630322664341812No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTACTTTGAGCAATG12740.1155774654787166No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT12740.1155774654787166No Hit
GAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCT12550.1138537827125505No Hit
GTCCCATGGTGGGTGAAAATCTATCCTGGATAACTCCTGGACCAGTACCA12340.111948659655209No Hit
CATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGA12300.11158577907285824No Hit
CCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCA12210.11076929776256904No Hit
AATGAGGACAGTGGGACCTAGATTAGACCATGAACGAGCCAAGTCCTTAA12210.11076929776256904No Hit
GATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGGACCTAGATTAGA12090.10968065601551678No Hit
CCCCCAGACCAGATGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG11840.10741265237582452No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT11620.10541680917289535No Hit
TTTTCATCCTGGGTGGCATGAACGGTCCCTCCAGAAAGAAGTCACTTCTC11430.10369312640672926No Hit
TCTTTGGTCCATTGTCAAGAAAAGCCTTGCGAGGAACCCAATGGTGTTCT11390.1033302458243785No Hit
AGCAGGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC11260.10215088393173853No Hit
ATCTAGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGAC11100.1006993616023355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)