FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_H06_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_H06_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500429
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC96270.6416164976816631No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTGTAGTAAGGACAATT39740.26485758406429094No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC36770.2450632452451932No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC34760.23166707654944022No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT28340.18887931384957235No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT28110.18734641892418769No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT28110.18734641892418769No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTATTGTCAGGACC27940.18621340963151206No Hit
AGCATATACTGACACATATCATTCCTATGCAAACAACATCCTAAGAACAC27440.1828810293589367No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA24990.16655236602331733No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG24380.16248686209077537No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA24340.16222027166896935No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA24200.16128720519264825No Hit
CCCCCAGACCAGATGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG22620.15075688353131006No Hit
TATCATTCCTATGCAAACAACATCCTAAGAACACAAGAAAGTGCCTGCAA22050.14695797002057412No Hit
AACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACA19040.12689704077967034No Hit
ATACTGAGGAGTGCACATGCGGATTTGCCAGCAATAAAACCATAGAATGT18870.12576403148699472No Hit
GGCGACATGGCAAATAGAATCCCCTTGGAATACAAAGGAATACAACTTAA18730.12483096501067362No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA18530.12349801290164346No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC18090.12056551826177712No Hit
GTTCTTTATTATTGTCATCCCTATTGCCATATCTGCTGATTCATTTACTC17320.11543365264201105No Hit
CAATAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACAT16800.11196797715853266No Hit
TTATAGAGCCATGTCGACACCTGTGACTGTGTCCCACAGCAGCTGTCCTG16740.11156809152582361No Hit
TCATAGATGTGCCCATACAGGCAGCAATTTCAACAACATTCCCATACACT16330.1088355397023118No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA16180.1078358256205392No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG16130.10750258759328166No Hit
GCATTATTGTCAGGACCATCAACTCCGATATATGTCCATTCCTTACCATC16050.1069694067496696No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT15870.10576974985154244No Hit
GGAATGATATGTGTCAGTATATGCTTCTCCATATTTTATTTTGAGCAATG15850.10563645464063945No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTGTAGTAAGGACAATT15560.10370367408254573No Hit
GTTATGATGAAGTATGTGCTTTTTCACACTTCATTGTTGAATGAAAGCAA15390.10257066478987009No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA15370.10243736957896707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)