FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_H06_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_H06_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135131
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC38940.3430441068035319No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC34430.3033130096878686No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC32710.28816057353732744No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA24530.21609840626324187No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA24040.21178172387151792No Hit
GCCAACAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGG23400.20614360809457236No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA22560.1987435811373313No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC22470.19795072110619832No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG20880.18394352722284915No Hit
CACTCTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAG20010.17627921358856377No Hit
GTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCCTAGCT19740.17390063349516488No Hit
GCAGGCAGGATGTGAGGTTGCTCCCTCTCCACCTGCTTCAGCCCTGCCAC17530.15443151495289972No Hit
GAGTTTATAAAATCTGATGGCCACGAATCTAATTTTATGAGACAGTGTAA16960.14941006808905757No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT16710.1472076791136882No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA16580.1460624368464961No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA16150.14227432780886085No Hit
CGTCCTAATAACAAGGGACCAATACATGGGTACCACACTGGGTGCTGAGA15890.1399838432744767No Hit
ATATGGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC15840.13954336547940283No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT15160.13355286746639813No Hit
GAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGTTTGA15100.13302429411230948No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA14600.12861951616157077No Hit
CTCAAAAGGCTGAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTG14240.1254480760370389No Hit
CTCTCTCACCCCACCTTCCTCTAGCATGCACCTGGCTGCCTTTGCCTCCC13820.12174806255841836No Hit
CTTATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAA13530.1191932913469899No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT13350.11760757128472397No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA13230.11655042457654667No Hit
ATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGT12920.11381946224708867No Hit
TTGCATGCCCCTCTTCATTCCTCAGGGCTCTGTCCTCAAACCATCCTTTT12460.10976706653240903No Hit
GGGACAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGT12270.10809325091112833No Hit
CTGTAGGGAAATCCAGGAGATAGTTAAACACAGGCAAGGGGCTGGAGCTC12250.10791705979309876No Hit
CTCATATACAGAGCTACAATCAACCAACACTCCCATCACCACCAACTGCC12230.10774086867506923No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC11830.10421704631447823No Hit
GTAAAGGCTAATGGAATGGGAGACGCCCTTGAACAGAAAGGCTTTCACCC11430.10069322395388726No Hit
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA11400.10042893727684295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)