FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_H05_FluA_Rep1_Plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_H05_FluA_Rep1_Plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634510
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACGA88550.5417525741659579No Hit
GTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATC85160.5210124135061883No Hit
GTCCTGGAGGGATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCA85070.5204617897718583No Hit
TCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAATGACTCCCACTTG59180.36206569552954704No Hit
GTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTGAAGAAAGACAGTTT56440.34530226183993984No Hit
CCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGGTATGAGT53990.33031306018317413No Hit
ATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTGCTGAGCAAGGCAGG53770.32896709105481153No Hit
CTCCAGGACAGTCATCCACCTCAGGTAGAAAGTTTCTGCCCTTTCCCCAC50000.3059020746278701No Hit
GATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAAAA43450.26582890285161914No Hit
GAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGT43040.2633205058396706No Hit
GCCTAGTAGTAGGAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCCA42190.25812017057099684No Hit
ATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCATGCTTAACACC41290.25261393322769515No Hit
GAGCAAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCT41240.25230803115306727No Hit
GCATTAGGCTGAGGGATGGGCCTAGTAGTAGGAATAAAAGAGGGATAAGA39920.2442322163828915No Hit
GTTTTAAGTACCGCTGATTTTCTTGCAGCTATTTGCCTAATTCCTCTGAC39190.23976604609332464No Hit
ACCATAAACACTGGAGATGGGGAATTATGAAATGGTATGAGTGGGGAAAG38270.2341374479201718No Hit
GTCTAAGCTCACACAGCCAGGTGGAGAGGAGCAGAGCTGAGATTCAAGCC37830.2314455096634465No Hit
GATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAG36310.2221460865947593No Hit
TTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATCACCAGCACCAATT35930.21982123082758745No Hit
GATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAG35480.21706811215593666No Hit
TGATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCA34750.21260194186636971No Hit
CTTAAAGACAGTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGG34110.20868639531113298No Hit
CCTATGAATCCCTCCAGGACAGTCATCCACCTCAGGTAGAAAGTTTCTGC33260.2034860600424592No Hit
GTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTGATTATACTGT32790.2006105805409572No Hit
GGCTAAGACAGGAACAGGCAGGACACAATTGTTACTTGACGGCCCTGCCT32600.1994481526573713No Hit
ATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGG32280.19749037937975294No Hit
AGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTC32240.19724565772005065No Hit
CCACAGAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAG32210.19706211647527394No Hit
GAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAA31390.19204532245137687No Hit
GGTATGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGT30600.1872120696722565No Hit
GGCCACAAGGTGGGAAGTAGGAGATAATGACTCCCACTTGTGTATTGATA30350.18568255929911717No Hit
GGATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGA29660.18146111066925255No Hit
CTTCTGTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTGATTAT28370.17356883714385352No Hit
TCATATCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTGCC28340.17338529589907678No Hit
GGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGA28300.17314057423937448No Hit
GGATGAGCCTGAGGCTCAGAATGGTGAAGCACCTTGTCTAAGCTCACACA28270.17295703299459775No Hit
GTTTATCACCAGCACCAATTCACAAGGATGAGCCTGAGGCTCAGAATGGT27300.16702253274681708No Hit
GTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTCC27270.16683899150204035No Hit
ACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACT26050.15937498088112034No Hit
GCATTCACCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGG25910.1585184550721623No Hit
GAATGGTGAAGCACCTTGTCTAAGCTCACACAGCCAGGTGGAGAGGAGCA25860.15821255299753442No Hit
GCTCCATACCCAGCACATAAACTTAATATTATTCCCCACACAGGCCACAA25690.15717248594379968No Hit
GTGGATGACTGTCCTGGAGGGATTCATAGGAGAGGCACATTTCAGGCCAA25510.15607123847513935No Hit
CTTCATATCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTG25400.15539825391095802No Hit
GTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGGTAATTACAGA24500.14989201656765636No Hit
TTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGCTAGTT24140.1476895216303357No Hit
ACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAG23460.14352925341539666No Hit
TTTCAGGCCAAGAGAGTTTCATGCTTAACACCACAGAGATATGAAGATCA23420.14328453175569436No Hit
GTGCAGCCAAGGCAGGTAGAGCAAAAGAGCATGAGTGCTGAAGTCTGACA23380.1430398100959921No Hit
GTGAAGCACCTTGTCTAAGCTCACACAGCCAGGTGGAGAGGAGCAGAGCT22880.1399807893497134No Hit
CTTAAGGGGAGGCAGGCATTAGGCTGAGGGATGGGCCTAGTAGTAGGAAT22790.1394301656153832No Hit
GGCCAAGAGAGTTTCATGCTTAACACCACAGAGATATGAAGATCACTGAG22310.13649350569895566No Hit
CTTGTGTATTGATAGGATAAATACCTTACTTAGTTCAGTTTATACAGCCT22180.13569816030492318No Hit
GCTTAACACCACAGAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAG21620.13227205706909104No Hit
TCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTGCCTCTCC21480.131415531260133No Hit
ACTTAATATTATTCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAAT21370.1307425466959517No Hit
TCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAAAAGAGCATGAGT21330.1304978250362494No Hit
GTGTTTATCACCAGCACCAATTCACAAGGATGAGCCTGAGGCTCAGAATG20960.12823414968400315No Hit
GTGTATTGATAGGATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGT20880.1277447063645986No Hit
CACTTAAGGGGAGGCAGGCATTAGGCTGAGGGATGGGCCTAGTAGTAGGA20130.12315617524518052No Hit
GTATTTATCCTATCAATACACAAGTGGGAGTCATTATCTCCTACTTCCCA20040.12260555151085034No Hit
CTTCAGCACTCATGCTCTTTTGCTCTACCTGCCTTGGCTGCACAGATGCC20000.12236082985114805No Hit
GTGCTGAAGTCTGACAAACCAGGGCTTGAATCTCAGCTCTGCTCCTCTCC19750.1208313194780087No Hit
ATTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTG19720.12064777823323199No Hit
GCCAGGTGGAGAGGAGCAGAGCTGAGATTCAAGCCCTGGTTTGTCAGACT19650.12021951532875297No Hit
ATATTATTCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAATGACTC19420.11881236578546477No Hit
GTTTATGGTCTTACAGGTGAATGCTTTGTCGTCTTCACCACCAGAACATC19380.11856764412576247No Hit
TCACCATTCTGAGCCTCAGGCTCATCCTTGTGAATTGGTGCTGGTGATAA19290.11801702039143229No Hit
CTTCTCTTCTGTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTG19260.11783347914665558No Hit
AGCTTAGACAAGGTGCTTCACCATTCTGAGCCTCAGGCTCATCCTTGTGA19070.11667105126306966No Hit
CACTGGAGATGGGGAATTATGAAATGGTATGAGTGGGGAAAGGGCAGAAA18970.11605924711381393No Hit
CTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATCAC18910.11569216462426048No Hit
GTCTTACAGGTGAATGCTTTGTCGTCTTCACCACCAGAACATCTGCTTAT18680.11428501508097227No Hit
GTGCTTCACCATTCTGAGCCTCAGGCTCATCCTTGTGAATTGGTGCTGGT18370.11238842221827948No Hit
ATTCACAAGGATGAGCCTGAGGCTCAGAATGGTGAAGCACCTTGTCTAAG18370.11238842221827948No Hit
GGGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTG18070.11055300977051226No Hit
GGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACGACAAAGC18050.11043064894066111No Hit
GTGGTAGTGTGTTCTCCACGTCATTTCTGTGACAATCAAATGAGATCACA17960.10988002520633094No Hit
CCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACGACAAAGCATTCA17840.10914586022722406No Hit
GCCCAATAAGCAGATGTTCTGGTGGTGAAGACGACAAAGCATTCACCTGT17730.10847287566304274No Hit
GAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGTCCTGG17720.10841169524811718No Hit
GTACTGAACAAGTAATTTCGGTGTGCTAGTTTAGAAGTGGGTTCGTATAG17640.10792225192871258No Hit
AACTAGCAACGTACTTAAAGACAGTATAATCACACCAAGCTCCTAGTAAA17610.10773871068393585No Hit
TCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCATGCTTAACACCAC17380.10633156114064765No Hit
CCATAAACACTGGAGATGGGGAATTATGAAATGGTATGAGTGGGGAAAGG17380.10633156114064765No Hit
ATTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGCTAGT17330.1060256590660198No Hit
CCTCTCCACCTGGCTGTGTGAGCTTAGACAAGGTGCTTCACCATTCTGAG17300.10584211782124306No Hit
GTATAAACTGAACTAAGTAAGGTATTTATCCTATCAATACACAAGTGGGA17230.10541385491676404No Hit
CAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCT17120.10474087035258273No Hit
GAATACTACCAGGTGTTTATCACCAGCACCAATTCACAAGGATGAGCCTG16970.10382316412869913No Hit
CAATTGTTACTTGACGGCCCTGCCTCTTTGGGCATAAAGCCCCGCACCTC16960.10376198371377354No Hit
GTATGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGTC16920.10351726205407126No Hit
GTACTGAACAAGTAATTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAG16840.10302781873466665No Hit
GAAGAAAGACAGTTTTAAGTACCGCTGATTTTCTTGCAGCTATTTGCCTA16720.10229365375555978No Hit
GTCATCCACCTCAGGTAGAAAGTTTCTGCCCTTTCCCCACTCATACCATT16670.10198775168093191No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)