FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_H04_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_H04_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGGCCCGCCATAGCAAACTGTAACACCAAGTGATTTTGATGATGAAG11.7543859649122806No Hit
GCCAAGCAACACCAACCTCTCCATCAAAGGCTTGTCAGGAACGTCCTTTG11.7543859649122806No Hit
TTTCTAGATACAAATACATATAACTGACATCACAATCATTCGATAGATCA11.7543859649122806No Hit
GCTATGACTTTCCTATAACAATCCCACAAAGGTATATATCACAAATCATG11.7543859649122806No Hit
GCCCTGAGCCGCAGAAGGAAACCAGGGGAGATTCTATAGTCTGGTGTGAG11.7543859649122806No Hit
CAAATACTCTCTTCTCCAAATTCAGAAAATTCTTCCCCTGGCCAAAAATA11.7543859649122806No Hit
ATTCAGCTCAGTGGAGCCAGGCATAGGAGAATCTCCCTGTGCCTGCACAG11.7543859649122806No Hit
GTAATGGTAAGAAATCTTTCGGGTAAGAGTATGGATGGTTCTTCTTTTAC11.7543859649122806No Hit
ATCTGACAGAGCAGAGGAGACGGGGAGAGAGCCAGTTGTAGGGAGGAACA11.7543859649122806No Hit
GTGGAAGATGGAATCGGGGAGACTCTGCCAGCAGAACATGTGGGCAAAGC11.7543859649122806No Hit
GCAAAAGCAGGTGCAGGATAAATGAAGGAACTGAACCAGCTAGGACCAAA11.7543859649122806No Hit
GGGACGTATATTGCATCTAATACACGGACCACGGTGTAGAGAAACAAGGG11.7543859649122806No Hit
GGAGGTTGCTGGTAACCTTGACAAGGGCTCTTCTTTAGTGTGTGATTGGG11.7543859649122806No Hit
TAAGAACCCTGGTGTTCCAATTTCCTCTCTCCCTTGAGCTCCATGGGCCC11.7543859649122806No Hit
GACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACA11.7543859649122806No Hit
GCCACGTCTGCTCTGCAGCGCAGCCTCTGCTAAGTGCCCCACTTGGACGT11.7543859649122806No Hit
GTTATCATGCGAACAAATCAACAGACACTCTAGACACAGTACTAGAAAAG11.7543859649122806No Hit
CTTTTATTGCTTGTGGACCAAATGAATGCAAACACTTTGCTCTAACCCAT11.7543859649122806No Hit
ATGCTCCTGTTATCCTGATTCTAGTTAAATCACATGTGTGTGCAGGGATA11.7543859649122806No Hit
CCTTTCTTCCCTTTCACTCCCTCTGGACAAACAGCAGATCCTTGCTTACC11.7543859649122806No Hit
GGCCTCAGAAGGACCAAAAAAGGTCATTTTTAAATGAAAGCTACAGCTTA11.7543859649122806No Hit
GCCCAAAGGAACAGAATATATGCTAATTTGCCTCCAAAAGAGAGGTCCCT11.7543859649122806No Hit
TACTGACACATATCATTCCTATGCAAACAACATCCTAAGAACACAAGAAA11.7543859649122806No Hit
GGCGTTAACATGAATGAAGCCAAGTTTATTGAAACTAAACCACGGACAAG11.7543859649122806No Hit
CACTGCAGCCCCCACCACCCATACTCAAGTGACACTCATAACACACCCAC11.7543859649122806No Hit
CTCCCACGCTGGGCCCACCGGGTGCGGGGTGCAGATCCTTGGGGCTGGGG11.7543859649122806No Hit
GAACCAGCTACGACCAAAAACACTAAATTGAACCAAACTTGATAGATACA11.7543859649122806No Hit
TTTCAGAAGCTATCACAGTAAACATGAAACTACAAATAAACCAACAGAAA11.7543859649122806No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCATTCATTACTCA11.7543859649122806No Hit
GGAAATTGCCCAATATGGGTGAAAACACCCTTGAAGCTGGCCAATGGAAC11.7543859649122806No Hit
GGCTCCCTCAGGAGTAACATAGCCCTCCTGTCTCTGACCCAGGGGAAGCA11.7543859649122806No Hit
TGATTGGATGACCAGGCAGAAGTCTGCTGCAAGGTCAGAACCATTATGGA11.7543859649122806No Hit
TAATCTAGTCGTGCATACATTCATAATCTATGGGCCATAGCTTCAAGCAT11.7543859649122806No Hit
GATGTGTCCCAGGGAGGTAAATTATTCCAAGCTCTCTGTTGGTCTTGCAA11.7543859649122806No Hit
GGGTGCTGAGACTTGGCCTTCTTCACACAAGTCCTCCTGTTAACAAATAA11.7543859649122806No Hit
GAGAGATTCTTAATAAAAATCACCAGATAGAGAAAAAATCAAAAATTAAA11.7543859649122806No Hit
CCTGGTGGGCTGTCACTGAACTTGGTTTCTGTTGTTCCTTCTTCTAAGAC11.7543859649122806No Hit
GACAGACCCTGAATCAGAGTCTCCAGCGGCCCATCGAGCTCAACGCACAG11.7543859649122806No Hit
ATTTGAGACATTTTAATTTTCTTTTGGTGTGCTTTCTCTGCATTTTCCCA11.7543859649122806No Hit
ATTTTTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATA11.7543859649122806No Hit
GAAAACCACCAATGAAAGAAGAGAAATCTATTTTCACAAAAGGTGGCATC11.7543859649122806No Hit
TGCACACTCCCTACCTCACAGACTCCTACTCAGACTCTGCCAGGCCTGGT11.7543859649122806No Hit
TATCTCTACAGGCACATTCTATGGTTTTATTGCTGGCAAATCCGCATGTG11.7543859649122806No Hit
AGCACGCACTTCAGTGATATTGTACATATACTCAACCTCAACGTCGACAG11.7543859649122806No Hit
CCCCAGCGCCTAATGCACACTCACAGGCGATTTCTGAGTGGTACCTTCTC11.7543859649122806No Hit
GTCTAGCTCCAAAAACAGCTCAAAGGTCTTCAGCACCCAGTTTCTCCATC11.7543859649122806No Hit
ACTGGTCACTCAGTGGCAGGGTCAGGCTAAGAAACCTGGTGTTCCAATTT11.7543859649122806No Hit
AAGGCCACCCAAGCAGATCGGAAAATTTCATTCTAAGCTGGTTTTACCTT11.7543859649122806No Hit
CGCAAGACTCCTCTGGGCCCGAGTGGGCTGAATCCCATGGGTTCAAAGCT11.7543859649122806No Hit
GAATGCCTGCATGTGCCAGGAAGCCTCAGCCACGCCAGCAAGGCCTCAAC11.7543859649122806No Hit
TTTGAGTGGTACTGGAGAAAGGCGGTGGTGTCTTGGTCTTCGTCTCCAGT11.7543859649122806No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG11.7543859649122806No Hit
GTATTAACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT11.7543859649122806No Hit
CTCATATACAGAGCTACAATCAACCAACACTCCCATCACCACCAACTGCC11.7543859649122806No Hit
CTTATAAGGCTGTGCACGCTGAGGCGACACAGAGGGTGAGCTCAGGGGCT11.7543859649122806No Hit
GTAGTAAGGAAAATTCTTCAAAAAAAATAGGAACAAAGGTTTGAGAACAG11.7543859649122806No Hit
CATTACCTGAGTGCCGGGCAGTGTGCCAAGCATGCTATGCAGCCTGCCAC11.7543859649122806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)