FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_H04_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_H04_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120373
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC25870.23090524316455324No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC25760.2299234272871624No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC19430.17342438634276264No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA18610.16610539525675824No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG16800.14995006127423635No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA15180.13549059107993497No Hit
GTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCCTAGCT14580.13013523174871225No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA14140.12620796823914893No Hit
ATATGGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC13850.12361954456239128No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA12840.11460468968816635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)