FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_H02_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_H02_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696474
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC21090.30281101663522253No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT18480.2653365380473643No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17200.2469582496977633No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT16770.2407842934553192No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT13640.19584363522543555No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC12050.1730143551661656No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG11860.17028632798927168No Hit
AGCATATACTGACACATATCATTCCTATGCAAACAACATCCTAAGAACAC11550.1658353362796027No Hit
GTATGAAAAGTCCTTCAAATAGCAACTGTTCGAAATACAATTGGACCGAT10940.15707693323799596No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTATTGTCAGGACC10500.15075939661782062No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA10420.14961075359597056No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG9910.1422881543316764No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT9780.14042160942117007No Hit
ATATAGACCCAAAAGGGGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC9500.13640135884469487No Hit
CCTAAGATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA9260.13295542977914465No Hit
TATCATTCCTATGCAAACAACATCCTAAGAACACAAGAAAGTGCCTGCAA9220.13238110826821964No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT9160.1315196260018321No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA9020.1295095007135945No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG8890.12764295580308813No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC8830.12678147353670058No Hit
CTCTTGAAGAGAGGAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA8770.12591999127031303No Hit
TCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGTTGCATTG8570.12304838371568788No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA8390.12046393691652524No Hit
GTTATAATGGGAGCTAATGACGTGAGTGAATTAGAATCACAAGCACAGCT8310.11931529389467518No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA8120.11658726671778129No Hit
ATACTGAGGAGTGCACATGCGGATTTGCCAGCAATAAAACCATAGAATGT8120.11658726671778129No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG8110.11644368634005002No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC7960.11428998067408115No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA7890.11328491802996235No Hit
TCATAGATGTGCCCATACAGGCAGCAATTTCAACAACATTCCCATACACT7670.1101261497198747No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA7520.10797244405390581No Hit
CTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCTTGAGTT7490.10754170292071204No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGGAAAG7480.1073981225429808No Hit
GGTCATAGGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG7420.10653664027659325No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT7370.10581873838793694No Hit
CCCCCAGACCAGATGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG7310.10495725612154941No Hit
AACCAATAGAGTAGTAAACGAAAAAGGAGAAAGTTTCGACATGCTTTACG7180.10309071121104305No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA7160.10280355045558054No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT7130.10237280932238677No Hit
GTAATATTCAGTGAATCAAAAGTGGGGAGAGAAAATTCTCCTGCATCAAA7020.10079342516734294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)