FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_G12_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_G12_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103872
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC37380.3386262175324675No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA22050.19975142045454544No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA21230.1923230229591837No Hit
GGCAAGGGGCTGGAGCTCAGGAGAGGCTTGGTCTGGAAGGAAAAAGTTGA18610.1685883870593692No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC18170.1646024176716141No Hit
GTGTAATGATAAAAGGAATGAGGGAGGCTGATAAATACAGATCTGTATGT17520.1587140538033395No Hit
GCCAACAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGG17270.15644929846938774No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA15490.1403242404916512No Hit
CACTCTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAG15220.1378783047309833No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA14080.12755102040816327No Hit
GATAAATACAGATCTGTATGTCTTCCAGTTTTAGCCAGCTTGTCTTGTTT13310.12057557397959183No Hit
GAGCAAAAGCAGGTGCAGGATAAATGAAGGAACTGAACCAGCTAGGACCA13120.1188543599257885No Hit
GAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGTTTGA12790.11586488288497217No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG12740.11541193181818182No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA12390.11224127435064934No Hit
CTCATATACAGAGCTACAATCAACCAACACTCCCATCACCACCAACTGCC12010.10879884624304266No Hit
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA11860.10743999304267161No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC11860.10743999304267161No Hit
CTCTCCACCTGCTTCAGCCCTGCCACTTACCCCAGTGAGCCTCTGCCCTT11240.10182339981447124No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT11230.10173280960111317No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA11060.10019277597402597No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)