FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_G08_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_G08_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725665
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC69080.40030944592374534No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA52420.3037669536091883No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT36480.21139676588445613No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC32540.18856498799013713No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA32150.1863049896706487No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATCATTGTCAGGGCC31490.18248037712997597No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT26690.1546650131978107No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT25500.14776912088962807No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG25400.14718963414104128No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT23400.13559989916930576No Hit
CCATTATACTTGACATACAGTCCCATCCCCATCCTTTCAGTCTTAGACAT22140.12829836613711235No Hit
TCATTGGATTGTGCAAACGCATCAAATGTTCAGGCTGTGAACCGTTCTGC21060.12203990925237518No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTACTTTGAGCAATG19900.11531786296876856No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT19390.112362480550976No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC18920.10963889283261816No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA18790.10888556005945534No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATACTAAGAACAC18590.10772658656228178No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC18410.10668351041482559No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT18330.10621992101595616No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG18280.10593017764166278No Hit
CCCCCAGACCAGATGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG18030.10448146077019585No Hit
TACCATTCCTATGCAAACAAAATACTAAGAACACAAGAAAGTGCCTGCAA17760.10291684654901154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)