FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_G05_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_G05_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585657
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG34240.21593572884930348No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT32780.2067281890093507No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT30990.19543949290420312No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC26850.16933044157721372No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC25410.16024903242000005No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA24150.15230279940743804No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG21530.1357796799686187No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT21430.13514902655492328No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA20230.12758118559057854No Hit
CCCATACACCGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA19580.12348193840155848No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC19520.12310354635334123No Hit
CCCCATAGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG19480.12285128498786307No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG19300.12171610884321137No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT19120.12058093269855966No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT18870.11900429916432116No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC18780.11843671109199531No Hit
GTGCTGACCTACTGGTTTCCTGTATGATGCACTGTTAAAACAGGCCTCAA18490.11660781619227867No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC17940.11313922241695398No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC17570.11080580478628102No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT17510.11042741273806378No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGA17330.10929223659341207No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT16800.10594977350082647No Hit
CCATAGAGGTTCTTCATTTGGGTTTTGTTATCTGAATGGAACCCCCAAAC16480.10393168257700121No Hit
ATGCATTACTGTCGGGGCCATCAACTCCGATATATGTCCATTCTTTACCA16480.10393168257700121No Hit
GTAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACGTAC16210.10222891836002364No Hit
CCTTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACT16150.10185052631180641No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA16130.10172439562906734No Hit
AAGCAGAACTAGCAGAAAAATTACACTGTTGGTTCGGTGGGAAAGAATTT16120.10166133028769778No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGTGTTCCTAGTT15950.1005892194844156No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)