FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_G05_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_G05_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675394
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC34980.20878670927554954No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC33920.20245983929750255No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC28870.1723176757228449No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA28410.169572052902183No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA24910.14868144448410345No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA19500.11639053261501472No Hit
CTCTCCACCTGCTTCAGCCCTGCCACTTACCCCAGTGAGCCTCTGCCCTT18960.11316741017336819No Hit
GCCAACAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGG17530.10463210444826711No Hit
CTCAAAAGGCTGAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTG17320.10337866794318232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)