FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_G04_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_G04_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2088892
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT55740.26684002811059643No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT46700.22356349682032386No Hit
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC44820.21456351022455925No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA38060.18220185629510766No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC36580.1751167604643993No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATCATTGTCAGGGCC33430.16003699568958088No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT28350.1357178829733658No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATACTAAGAACAC26240.12561683418769376No Hit
CCCCCAGACCAGATGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG26110.12499449468905047No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT24340.1165211030536763No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA23020.1102019635289905No Hit
CCATTATACTTGACATACAGTCCCATCCCCATCCTTTCAGTCTTAGACAT22210.10632430972975146No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT22120.1058934593076138No Hit
CTGTTATCTCTACAGGCACATTCTATGGTTTTATTGCTGGCAAATCCGCA21640.10359559038954623No Hit
TACCATTCCTATGCAAACAAAATACTAAGAACACAAGAAAGTGCCTGCAA21170.10134559374060507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)