FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_G03_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_G03_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962073
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT50850.25916466920445874No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA43590.22216298781951538No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG36860.18786253110867945No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCACTCTGCTGT35380.18031948862249264No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC29300.1493318546251847No Hit
TCTCTGTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCA28050.14296104171455395No Hit
CTGTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGC27080.13801729089590448No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG26610.13562186524150732No Hit
GAATAGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAAAAAG26570.13541799922836714No Hit
ATTCAGGACCTTGAGAAATATGTTGAGGACACTAAAATAGATCTCTGGTC24130.12298217242681592No Hit
CAATAGGATGGGGGCTGTAACCACCGAAGTGGCATTTGGCCTGGTATGTG23470.11961838321000288No Hit
GAGTAAGGAATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA23310.11880291915744216No Hit
AATCAATGGAAAGCTGAATAGATTGATCGGGAAAACCAACGAGAAATTCC22380.11406303435193288No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG22290.11360433582236747No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT22160.11294177127966187No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG22070.11248307275009645No Hit
TCTCAAAAGCACTCAAGCAGCAATCGATCAAATCAATGGAAAGCTGAATA21470.10942508255299369No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC20400.10397166670149378No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCACTCT19680.10030207846497047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)