FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_G02_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_G02_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787184
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA32550.4134992581150024No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG18300.23247423728124555No Hit
ATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAAT13160.16717819467875364No Hit
AACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACC12030.15282322811439256No Hit
AGTAGAAACAACGCACTTTACTGAAGAATCTAAGCTGGAACAAATAATAT11780.1496473505559056No Hit
CTTCATAATCAGAACAAACATGTGATTGAGTGGAACAAGCTAGTGCTAGC11700.14863106973718979No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG10110.1284324884652127No Hit
TTACTGAAGAATCTAAGCTGGAACAAATAATATGAAGTTTCTCACATTCT9570.12157259293888087No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG9360.11890485578975182No Hit
ACACAATACCTTCCCACAATAGCCACTTCATAATCAGAACAAACATGTGA8980.11407752190085166No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT8300.1054391349417671No Hit
ATTTTGTAGTGATGGAGGGCAGTTGAATAATTTTAAAAAGGGGAATTACA8250.10480395943006972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)