FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_G02_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_G02_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060684
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA52350.2540418618284026No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT46460.22545911939918978No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA45490.22075194449998153No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG44990.2183255656859567No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA44730.21706384870266376No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA36430.17678596038985114No Hit
ATATGGGATCGAGTACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG31870.15465738560594444No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC30250.1467959182485039No Hit
ATCACGTACTGTGTTATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC29390.14262254668838115No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG28070.1362169066193555No Hit
GTTCAAGAGTACTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT26420.12820985653307349No Hit
TCTCTGTACACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCA26240.12733636016002453No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG25660.12452176073575572No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCGT25570.12408501254923122No Hit
CTCAATATCAATGCGGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC25110.12185274404032835No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT24900.12083366493843792No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT24780.12025133402307196No Hit
CTCATAAGCACTTCTTGTTTTCCGCCCATTCTCACCTCTCCAAAAATTTC24610.1194263652263035No Hit
GAGTAAGGAATATCCCTAGCAGAGTAAGCATCTATTGGACAATAGTAAAA24240.11763084490392511No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAGAAAAAGACG23250.1128266148521559No Hit
ATCCAGAACAGCTTGACAATAGAGAAAATGGTACTCTCTGCTTTTGATGA22330.10836207783435015No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT22310.10826502268178916No Hit
ATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTTGACAG22210.10777974691898419No Hit
GGTCTATTCCCACCAAGGAATATCCCTCTTTTTCGAAGTCGTACCCACTG22090.1071974160036182No Hit
GGATACTGCAGGTCCATACGCACAGGCAGGTAGGCAAGATTTGTGAGCAA21980.10666361266453274No Hit
ATCCAGTGGGTACGACTTCGAAAAAGAGGGATATTCCTTGGTGGGAATAG21940.10646950235941076No Hit
GTACGTGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT21540.10452839930819087No Hit
CCCATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTA21250.10312109959605645No Hit
GCAGTATCCAGTGGGTACGACTTCGAAAAAGAGGGATATTCCTTGGTGGG21250.10312109959605645No Hit
GCTTATGAGAGAATGTGCAACATTCTTAAAGGAAAATTTCAAACAGCTGC21220.10297551686721497No Hit
GATCAGAATGGTCAAACGGGGGATCAACGATCGAAATTTTTGGAGAGGTG21050.10215054807044652No Hit
GAATAGACCCTTTCAAACTACTTCAAAATAGCCAAATATACAGCTTAATC20960.10171379988392203No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)