FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n01_Flu09_F08_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n01_Flu09_F08_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16673
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTAGGTGTGATCGTCAAGGAACCGATACCTTTAGCTGAGGTCTCTGG590.3538655310981827No Hit
ACCATGGACAGGCCCGCTTCAATAGCAGCGAGGTCCTCAGCGTTGCCGAT500.2998860433035446No Hit
CTTTACGGGAGTGCATTCTATGCTGAAACAAATTCAACATTACATTGACA490.29388832243747376No Hit
TCATAGCGGTGACCCCGCGATACCCATTCGACCAGAACCATTGGACGCCC480.2878906015714029No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTA470.28189288070533197No Hit
GATGTAGAGGCACCACCACAGACCCAGATGCTCGCCCAGGCTGAAGTGAA450.2698974389731902No Hit
ATACAGCACGGTCCCCTTTATGGCACAAGTCCACATAGAGGCGCGGCTTC430.2579019972410484No Hit
CAATAACACGGAGTGCCCTATGGGGTTGAACCTTATACCTTAAAGATCAC420.25190427637497753No Hit
ATCTCATAGCGTCCAGTGTCGAGACGCTTCGTGTAGCCCATGACCTTAAA380.22791339291069393No Hit
CCTTACAAGTTGATGCCAGTGCCCCCGGAACGGGAAACACTCAAGCCACC370.22191567204462304No Hit
CTATGAGATTAACTACGTGAAAGTTTAGGAGGCCCTCATGGGTAAGAAAG360.21591795117855214No Hit
ACCTAAGGGTGTCGAAGTCGGTGATGGCCCAGCAGATGGTGATGATGCAC360.21591795117855214No Hit
CCCTTAGGTGGTTCGAGTAGCTGTGGCTCAGGCGCCTGCAACTGTTGAGG350.20992023031248128No Hit
CTCATAAGGTGCCCGGTCGGTTTTCAGACGGTCATATGCCGCCTTAGCTC350.20992023031248128No Hit
GGTCTCATAAGGTGCCCGGTCGGTTTTCAGACGGTCATATGCCGCCTTAG320.19192706771426857No Hit
ATCTTAAAGTACGTCTGAACGCTGCCTATCTGGAACGCACCGGGGTCCTT320.19192706771426857No Hit
ACATAGAGGCGCGGCTTCATAGCTTGAGAGCTCGCTTGATGGCAGGAGAC320.19192706771426857No Hit
GCCTTGAAGGAATCCGAGGATCTTCCCTTCAGACCAGCCCGCACGTTGTA310.18592934684819767No Hit
CACCTAAGGGTGATGGGTCTACCAAGGCTGTAGCCAAGGATACTGGCGCG310.18592934684819767No Hit
GCCTATCTGGAACGCACCGGGGTCCTTGACGACCTACAACGTGCGGGCTG310.18592934684819767No Hit
CCCGTAAAGTGATCTTTAAGGTATAAGGTTCAACCCCATAGGGCACTCCG300.17993162598212678No Hit
GTTTTGTGCACGGGTCTCATAAGGTGCCCGGTCGGTTTTCAGACGGTCAT300.17993162598212678No Hit
GTATGGTCTCCCTGGCGGCGTCTCAAGGAATGACCGAGGAAGACCTTCTG290.1739339051160559No Hit
TATGAGACCCGTGCACAAAACTGTGCCAAAGTAACAATCGGTTCTCTGTT290.1739339051160559No Hit
GGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCCG290.1739339051160559No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGTGCCCGGGTTATTA280.16793618424998502No Hit
TCCCTGAGATGACCATGCAGTCCACACACCGAGAAGCTCATGCACGCATC280.16793618424998502No Hit
GTCATATGCCGCCTTAGCTCCTTCCTCAGCGAGACCCTTTCGGGCTTCTG280.16793618424998502No Hit
ATGTAGGACATCATGACCCGCTCAACCATGGACAGGCCCGCTTCAATAGC280.16793618424998502No Hit
CCCATGAGTGGACCCACATGGCTATCATCGTCCTCGGTGAGAACAGCGAA270.1619384633839141No Hit
GTATAAGGTTCAACCCCATAGGGCACTCCGTGTTATTGTGAGGGGTTTTA270.1619384633839141No Hit
CCTTTACCGCCACGCTTTGCAGCCTTGCGTGCAGCATCGGTTTCTTTGTC270.1619384633839141No Hit
GTGCTGTAGCTGGTCGAGCTGTTGTCGGACTCAGCAGGGAACAGAGAACC260.15594074251784323No Hit
GCCCTACAGTGGTTATCGCTCAGGTGTCCACAGGATCGGCTCTTCTTTCT260.15594074251784323No Hit
AGTCCACATAGAGGCGCGGCTTCATAGCTTGAGAGCTCGCTTGATGGCAG250.1499430216517723No Hit
GCGTAAGGGTGAGTTCGAGACTCGTACACGGGTCACCGAGAAACCTGAAG250.1499430216517723No Hit
GCTTCATAGCTTGAGAGCTCGCTTGATGGCAGGAGACTTGGTGGTGCTGG250.1499430216517723No Hit
CCTACAAGGGCAACCGTGTAGCCAAGCGCAAGCCGGTGGGTTACCCAGCG250.1499430216517723No Hit
AAAGGGGACCGTGCTGTATTCCGTGAGAAGCTCGACGAGATCATTGCGCG250.1499430216517723No Hit
CCTTAAAGCCGTTCTCTACGGCCAGCTCACGAGCCCTTCGGTGCATCATA240.14394530078570145No Hit
GTGTCGAGACGCTTCGTGTAGCCCATGACCTTAAAGCCGTTCTCTACGGC240.14394530078570145No Hit
TCTCAGGGAAGCGCGCAATGATCTCGTCGAGCTTCTCACGGAATACAGCA240.14394530078570145No Hit
CCTAAGGGTGATGGGTCTACCAAGGCTGTAGCCAAGGATACTGGCGCGAC240.14394530078570145No Hit
GTGGTGGCGGTGGTGGTAAGGCCCCTGATGTAGAGGCACCACCACAGACC230.13794757991963055No Hit
CCTTTAGCTGAGGTCTCTGGTGCATCATCACCATCTGCTGGGCCATCACC230.13794757991963055No Hit
ACCTTATACCTTAAAGATCACTTTACGGGAGTGCATTCTATGCTGAAACA230.13794757991963055No Hit
ATGTGGACTTGTGCCATAAAGGGGACCGTGCTGTATTCCGTGAGAAGCTC230.13794757991963055No Hit
ACATAGGTCAGTCCTCCTGTTCTTCAAATTTCTCAGGGTTCTGCATCAGC230.13794757991963055No Hit
ATTCTATGCTGAAACAAATTCAACATTACATTGACAATCCCGAAGATGTT230.13794757991963055No Hit
CTTCAAGGCTGTGCAGCAGTAACCGAGATCATCGAGCTGATGCAGAACCC220.13194985905355963No Hit
CTCGTACACGGGTCACCGAGAAACCTGAAGAGGCCAGCTTGTGGGACTGT220.13194985905355963No Hit
GAATACAGCACGGTCCCCTTTATGGCACAAGTCCACATAGAGGCGCGGCT220.13194985905355963No Hit
GCTCCAGAGGGAACAACGCAAGGTGGACTTTGGCAGCGAGGTTGTTGACG220.13194985905355963No Hit
GTGTTATTGTGAGGGGTTTTAAACCTCCACACAGAATGCCCTACAGTGGT220.13194985905355963No Hit
GCTGTATTCCGTGAGAAGCTCGACGAGATCATTGCGCGCTTCCCTGAGAT220.13194985905355963No Hit
GAGATTAACTACGTGAAAGTTTAGGAGGCCCTCATGGGTAAGAAAGTCAA210.12595213818748877No Hit
CTTCAATAGCAGCGAGGTCCTCAGCGTTGCCGATCAGTTGCTTGGCTTGC210.12595213818748877No Hit
CCTCTATGTGGACTTGTGCCATAAAGGGGACCGTGCTGTATTCCGTGAGA210.12595213818748877No Hit
GTGTACGAGTCTCGAACTCACCCTTACGCTTACCGCGCAGAAGTTCATGC210.12595213818748877No Hit
CCTCATGGCGTGGCTCGACGAACCCAAGATGTACGAGAAGTATGAGCATG210.12595213818748877No Hit
GGCTCAAAGTTATCAGTCGGACTACGTGCGTAGGCACATCCGGCTGGTCA200.11995441732141786No Hit
CCCTTACGCTTACCGCGCAGAAGTTCATGCTCATACTTCTCGTACATCTT200.11995441732141786No Hit
CCGCTATGATGCACCGAAGGGCTCGTGAGCTGGCCGTAGAGAACGGCTTT200.11995441732141786No Hit
GCTTGATACGGAGTGCTGTAGCTGGTCGAGCTGTTGTCGGACTCAGCAGG200.11995441732141786No Hit
AAGCAGAGCGGCCCCGGCAATTACGAGCTGACGAATCAGTTCATGCAGGG200.11995441732141786No Hit
GTGCTGTATTCCGTGAGAAGCTCGACGAGATCATTGCGCGCTTCCCTGAG190.11395669645534696No Hit
GTATGAGCATGAACTTCTGCGCGGTAAGCGTAAGGGTGAGTTCGAGACTC190.11395669645534696No Hit
GGTATAAGGTTCAACCCCATAGGGCACTCCGTGTTATTGTGAGGGGTTTT190.11395669645534696No Hit
CCTTAAAGATCACTTTACGGGAGTGCATTCTATGCTGAAACAAATTCAAC190.11395669645534696No Hit
ATGCAGGGTCGTTCGGTAACTGTTGGACTCCATGTAGGACATCATGACCC190.11395669645534696No Hit
ACTCATGGGCGTCCAATGGTTCTGGTCGAATGGGTATCGCGGGGTCACCG190.11395669645534696No Hit
ATGATGCACCGAAGGGCTCGTGAGCTGGCCGTAGAGAACGGCTTTAAGGT180.10795897558927607No Hit
CAGGAGGACTGACCTATGTGTTTCGGGTCCAAGATGAAAGTTCCCAAACC180.10795897558927607No Hit
GCCCTATGGGGTTGAACCTTATACCTTAAAGATCACTTTACGGGAGTGCA180.10795897558927607No Hit
GGGTTGAACCTTATACCTTAAAGATCACTTTACGGGAGTGCATTCTATGC180.10795897558927607No Hit
CCGCCACCACTGTCTGAGAACCCGCTACCGGGGATCTTGCTGGCAACCTT180.10795897558927607No Hit
ATCTGGGTCTGTGGTGGTGCCTCTACATCAGGGGCCTTACCACCACCGCC180.10795897558927607No Hit
CCTACGGCTTGATACGGAGTGCTGTAGCTGGTCGAGCTGTTGTCGGACTC180.10795897558927607No Hit
GTGTGTGGACTGCATGGTCATCTCAGGGAAGCGCGCAATGATCTCGTCGA180.10795897558927607No Hit
GCTTTAAGGTCATGGGCTACACGAAGCGTCTCGACACTGGACGCTATGAG180.10795897558927607No Hit
CCACAGACCCAGATGCTCGCCCAGGCTGAAGTGAAGACTGACGAAGCGAC180.10795897558927607No Hit
CTTTAAGATAGTCAGCAGACGCTGTTGGAATGTCAGGAACATCTTCGGGA180.10795897558927607No Hit
GTTTTAAACCTCCACACAGAATGCCCTACAGTGGTTATCGCTCAGGTGTC180.10795897558927607No Hit
GTGTAGCCCATGACCTTAAAGCCGTTCTCTACGGCCAGCTCACGAGCCCT180.10795897558927607No Hit
CCATGTAGGACATCATGACCCGCTCAACCATGGACAGGCCCGCTTCAATA180.10795897558927607No Hit
ATTACATTGACAATCCCGAAGATGTTCCTGACATTCCAACAGCGTCTGCT180.10795897558927607No Hit
GCTTGTGGGACTGTATGGTCTCCCTGGCGGCGTCTCAAGGAATGACCGAG170.10196125472320518No Hit
ATCTGGAACGCACCGGGGTCCTTGACGACCTACAACGTGCGGGCTGGTCT170.10196125472320518No Hit
CTGTAGGGCATTCTGTGTGGAGGTTTAAAACCCCTCACAATAACACGGAG170.10196125472320518No Hit
CGTTGTAGGTCGTCAAGGACCCCGGTGCGTTCCAGATAGGCAGCGTTCAG170.10196125472320518No Hit
GTATTCCGTGAGAAGCTCGACGAGATCATTGCGCGCTTCCCTGAGATGAC170.10196125472320518No Hit
GTGCAGCAGTAACCGAGATCATCGAGCTGATGCAGAACCCTGAGAAATTT170.10196125472320518No Hit
TTCTTACCCATGAGGGCCTCCTAAACTTTCACGTAGTTAATCTCATAGCG170.10196125472320518No Hit
ATCATGTACGGCACCATCAAGGGGATCAAGAAGGACATCGCCAAGCAACT170.10196125472320518No Hit
GTTAATCTCATAGCGTCCAGTGTCGAGACGCTTCGTGTAGCCCATGACCT170.10196125472320518No Hit
TGGTAGACCCATCACCCTTAGGTGTGATCGTCAAGGAACCGATACCTTTA170.10196125472320518No Hit
TAGTTAATCTCATAGCGTCCAGTGTCGAGACGCTTCGTGTAGCCCATGAC170.10196125472320518No Hit
GTACGCCACCGTAGCCATCAGTCGTGTCGCCCATGAGGGTTTGACGCATG170.10196125472320518No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)